气相色谱法测定艾叶4个挥发性成分的含量

目的: 测定不同产地艾叶中桉油精、侧柏酮、樟脑、龙脑4个挥发性成分的含量。 方法: 采用气相色谱程序升温法测定。使用安捷伦HP-5毛细管柱(30 m×0.32 mm×0.25 μm),载气为高纯氮气(99.99%),流速0.6 mL·min^-1,分流比为10∶1,进样口温度240 ℃,进样量1 μL,检测器温度250 ℃,程序升温(起始温度50 ℃,以4.0 ℃·min^-1升至75 ℃,保持10 min,以1.0 ℃·min^-1升至100 ℃,以8.0 ℃·min^-1升至150 ℃,保持2 min,以3.0 ℃·min^-1升至165 ℃,保持5 min,以8.0 ℃·min^-1升至250 ℃,保持3 min)。 结果: 以该方法测定桉油精、侧柏酮、樟脑、龙脑的回收率在95%~103%之间。各产地艾叶样品均含有桉油精、樟脑、龙脑,部分样品含有侧柏酮。 结论: 该方法可为艾叶质量标准的提高提供科学数据。

Objective: To determine the contents of four volatile components(eucalyptol,thujone,camphor and borneol)of Artemisia argyi from different origins. Method: The essential oil of Artemisia argyi from different origins was extracted by steam distillation,and quantitatively determined by GC linked with Agilent HP-5 capillary column(30 m×0.32 mm×0.25 μm).The carrier gas was high purity nitrogen at a flow rate of 0.6 mL·min^-1.The inlet temperature was 240 ℃,the injection volume was 1.0 μL,and the FID detector temperature was 250 ℃.The column temperature was raised by program:the initial temperature was 50 ℃,raised to 75 ℃ with a rate of 4 ℃·min^-1,maintained for 10 min,then raised to 100 ℃ with a rate of 1 ℃·min^-1,raised to 150 ℃ with a rate of 8 ℃·min^-1,maintained for 2 min,again raised to 165 ℃ with 3 ℃·min^-1,maintained 5 min,and the last raised to 250 ℃ with the rate of 8 ℃·min^-1,maintained 3 min. Results: The recoveries of eucalyptol,thujone,camphor and borneol were between 95%-103%.All samples contained eucalyptol,camphor and borneol,and some of them contained thujone. Conclusion: The method can contribute to the improvement of Artemisia argyi quality standard by providing scientific data.