离子液体敏化分光光度法测定依巴斯汀片中依巴斯汀含量

目的: 建立一种测定依巴斯汀含量的新方法。 方法: 用光谱法研究依巴斯汀、水杨基荧光酮及1-丁基-3-甲基咪唑四氟硼酸盐(C₄)间的相互作用。利用在pH6.5的醋酸缓冲体系中, 依巴斯汀与水杨基荧光酮 反应能形成稳定的化合物,且C₄离子液体对其具有增敏作用,该物质在560 nm波长处有最大吸收,且其吸光度与依巴斯汀浓度在一定范围内存在定量关系,建立了测定依巴斯汀含量的新方法。 结果: 在优化条件下,线性方程为: A＝0.029+0.005C (C,μg·mL^-1), r＝0.9995,检出限为 0.96 μg·mL^-1(S/N=3),线性范围为2.5~20.0 μg·mL^-1,回收率为96.1%~106.4%。 结论: 本法快速准确,简便易行,适用于依巴斯汀片中依巴斯汀含量的测定。

Objective: To establish a method for the determination of ebastine (EBS). Method: The interaction of EBS,salicyl fluorine (SAF) and 1-butyl-3-methylimidazolium tetrafluoroborate (C₄) was studied by spectrophotometry.It showed a steady compound was formed through the interaction of EBS and SAF in the acetic acid buffer system (pH=6.5) and the interaction could be sensitized by C₄.The compound had a maximum absorption at 560 nm and there was a good linear relationship between the absorbance of the compound and a certain concentration range of ebastine concentration. Results: The equation of calibration curve was A＝0.029+0.005C (C:μg·mL^-1).The range of calibration curve was between 2.5 and 20.0 μg·mL^-1 (r=0.9995) and the detection limit was 0.96 μg·mL^-1(S/N=3).The recovery was 96.1%-106.4%. Conclusion: The method is simple,rapid with good accuracy for determining ebastine.

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