RP-HPLC测定多叶棘豆全草中2个黄酮苷含量

目的: 建立多叶棘豆全草中2个黄酮苷类化合物异鼠李素-3-O-α-L-吡喃鼠李糖基-(1→2)[O-α-L-吡喃阿拉伯糖基-(1→6)]-O-β-D-吡喃葡萄糖苷(WS-3)和异鼠李素-3-O-α-L-吡喃阿拉伯糖基-(1→6)-O-β-D-吡喃葡萄糖苷(WS-16)的含量测定方法。方法:采用RP-HPLC方法,使用SunFire C₁₈色谱柱(150 mm×4.6 mm,5 μm),以乙腈-0.1%甲酸水(17∶83)为流动相,流速1.0 mL·min^-1,检测波长350 nm,柱温30 ℃。结果:WS-3和WS-16的线性范围分别为0.045~0.226 μg(r=0.9998,n=5)和0.042~0.21 μg(r=0.9997,n=5);平均回收率(n=6)分别为101.1%(RSD=1.6%)和100.5%(RSD=2.2%)。结论:适用于多叶棘豆药材的质量控制和评价。

Objective: To establish a method for the content determination of two flavonoid glycosides,isorhamnetin-3-O-α-L-rhamnopyranosyl-(1→2) [O-α-L-arabinopyranosyl-(1→6)]-O-β-D-glucopyranoside(WS-3) and isorhamnetin-3-O-α-L-arabinopyranosyl-(1→6)-O-β-D-glucopyranoside(WS-16),in the whole herbs of Oxytropis myriophylla (Pall.) DC.. Methods: The RP-HPLC separation was performed on a SunFire C₁₈ analytical column(150 mm×4.6 mm,5 μm),and the mobile phase was acetonitrile-water containing 0.1% formic acid(17∶83) at a flow rate of 1.0 mL·min^-1.The detection wavelength was set at 350 nm and the column temperature was 30 ℃. Results: The linear ranges of WS-3 and WS-16 were 0.045-0.226 μg(r=0.9998,n=5) and 0.042-0.21 μg(r=0.9997,n=5),respectively.The average recoveries(n=6) were 101.1%(RSD=1.6%) and 100.5%(RSD=2.2%),respectively. Conclusion: The method can be used for quality control and evaluation of the O.myriophylla herbs.

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