不同来源、不同等级黄芪饮片中毛蕊异黄酮葡萄糖苷含量分析

目的: 为制订黄芪饮片等级标准提供研究基础。 方法: 采用HPLC法测定52批不同来源、不同等级的黄芪饮片中毛蕊异黄酮葡萄糖苷含量。使用Agilent C₁₈色谱柱(4.6 mm×250 mm,5 μm),流动相为乙腈-0.2%甲酸水溶液,梯度洗脱,流速1.0 mL·min^-1,检测波长260 nm,柱温30 ℃。 结果: 52批不同来源、不同等级黄芪饮片中毛蕊异黄酮葡萄糖苷含量与饮片直径大小和等级不呈正相关。 结论: 以毛蕊异黄酮葡萄糖苷含量测定为指标之一,为制订黄芪饮片等级标准提供参考依据。

Objective: To provide the basis for studying the grade standard of medicinal slices of Radix Astragali. Methods: The content of calycosin glucoside in 52 batches of Radix Astragali of various grades and different sources was determined by HPLC.The analysis was performed on an Agilent C₁₈(4.6 mm×250 mm,5 μm)column.The mobile phase is methanol-0.2% formic acid aqueous solution by gradient elution,at the flow rate of 1.0 mL·min^-1.The detection wavelength was 260 nm and the column temperature was 30 ℃. Results: The contents of calycosin glucosides in the 52 batches of the samples had no positive correlation with the size and grade of the samples. Conclusion: Scientific basis for drawing up quality standard of medicinal slices of Radix Astragali at different grades is provided,taking the content determination of calycosin glucoside as one of the quality indexes.

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