高效液相色谱串联质谱法分析紫红参中人参皂苷类成分

目的: 采用高效液相色谱串联质谱(LC-MS/MS)技术对纳米级紫红参及普通紫红参中皂苷类成分进行分析研究,为进一步开发发酵紫红参产品奠定基础。 方法: 纳米紫红参粉和普通紫红参粉用甲醇进行索氏提取,浓缩,通过0.22 μm滤膜过滤。液相色谱采用Agilent XDB C₁₈(3.0 mm×100 mm,1.8 μm)色谱柱,以0.05%甲酸水溶液-甲醇为流动相,梯度洗脱(0~0.5 min,20%B;0.5~5 min,20%B→50%B;5~45 min,50%B→80%B;45~50 min,80%B→100%B;50.1~55 min,20%B),流速0.4 mL·min^-1,柱温55 ℃。质谱采用电喷雾电离,负离子,多反应监测(MRM)模式检测,分析测定紫红参中皂苷类成分的含量。 结果: LC-MS/MS法测定的纳米级紫红参中含有57个人参皂苷,普通紫红参中含有30个,其中纳米级紫红参中含有2种人参皂苷元和2个乙酰化人参皂苷,而普通级紫红参中则没有检测到。 结论: 紫红参中稀有人参皂苷含量较高,其中纳米级紫红参在皂苷种类及含量上较普通级紫红参多。

Objective: To analyze and study the contents of saponins in the nanometer black red ginseng and the common black red ginseng by HPLC-MS/MS and lay a foundation for further development of the product of fermented black red ginseng. Methods: Saponins in the nanometer black red ginseng and the common black red ginseng were extracted by methanol with the Soxhlet apparatus,then were concentrated and filtered by 0.22-μm membrane.An Agilent XDB C₁₈(3.0 mm×100 mm,1.8 μm) column was used for liquid chromatography,with 0.05% aqueous formic acid and methanol as the mobile phase for gradient elution (0-0.5 min,20%B;0.5-5 min,20%B→50%B;5-45 min,50%B→80%B;45-50 min,80%B→100%B;50.1-55 min,20%B),with a flow rate of 0.4 mL·min^-1,and a column temperature of 55 ℃.The mass spectrometry detection was performed with electrospray ionization and negative ion in multiple reaction monitoring (MRM) mode to determine the contents of saponins in black red ginseng. Results: Fifty-seven ginsenosides in the nanometer black red ginseng and 30 ginsenosides in the common black red ginseng was determined by LC-MS/MS,respectively.Among them,2 kinds of ginseng aglycone and 2 acetylated ginsenosides were determined in the nanometer black red ginseng,while none was detected in the common black red ginseng. Conclusion: There are more rare ginsenosides in the black red ginseng,and there are more types and higher contents of saponins in the nanometer black red ginseng than those in the common black red ginseng.