中药复方三妙丸中化学成分的HPLC-TOF/MS分析

目的: 采用高效液相色谱-飞行时间质谱(HPLC-TOF/MS)联用技术分析中药复方三妙丸中的主要化学成分。 方法: 色谱分离采用SHISEIDO CAPCELL PAK C₁₈色谱柱(3.0 mm×100 mm,3 μm),流动相为乙腈(A)-0.1%甲酸水溶液(B),梯度洗脱,流速0.6 mL·min^-1,柱温25 ℃。质谱定性鉴别采用TOF/MS正离子模式扫描,电压4 kV,雾化气压力275.85 kPa,干燥气流速10 L·min^-1,干燥气温度350 ℃。 结果: 在优化的液质联用条件下,通过飞行时间质谱以及碎片离子裂解分析,鉴定了三妙丸中23个化学成分,包括3组同分异构体。 结论: 所建立的HPLC-TOF/MS联用法,可为鉴定三妙丸中的化学成分提供一种快速、高效的方法。

Objective: To identify the main chemical components in compound Sanmiao pills by high performance liquid chromatography–time-of-flight mass spectrometry (HPLC-TOF/MS). Methods: A SHISEIDO CAPCELL PAK C₁₈ column (3.0 mm×100 mm,3 μm) was used for separation and identification of chemical components in Sanmiao pills with a mobile phase of acetonitrile (A) and 0.1% formic acid in water solution (B) with gradient elution.The flow rate was set at 0.6 mL·min^-1 and the column temperature was set at 25 ℃.Time-of-flight mass spectrometer (TOF/MS) was applied for qualitative analysis under positive ion mode.The capillary voltage was set to 4 kV,the nebulizer gas pressure was 275.85 kPa,the drying gas flow rate was set to 10 L·min^-1 at a temperature of 350 ℃. Results: Under optimized LC/MS conditions,23 chemical constituents of Sanmiao pills including 3 pairs of isomers were identified through analyses of time-of-flight mass spectrometer and fragment ion splitting. Conclusion: A rapid and efficient HPLC-TOF/MS approach has been established for studying the chemical constituents in Sanmiao pills.

1 ChP(中国药典).2010.Vol Ⅰ(一部):454
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