顶空固相微萃取气质联用法分析阿育魏实挥发性成分

目的: 利用顶空-固相微萃取(HS-SPME)结合气相色谱-质谱联用技术(GC-MS)分析阿育魏实挥发性化学成分,为其挥发性成分分析提供新方法。 方法: 实验中以PDMS、CAR/PDMS、PDMS/DVB和DVB/CAR/PDMS为微萃取纤维头,顶空固相微萃取方法提取阿育魏实中挥发性成分,采用GC-MS对挥发性成分进行分离鉴定,面积归一化法确定各成分的相对质量分数,并与传统的水蒸气蒸馏法(HD)提取的挥发性成分进行了比较。 结果: DVB/CAR/PDMS萃取纤维有效地萃取阿育魏实挥发性成分,其主要成分为γ-萜品烯(26.21%)、p-伞花烃(23.58%)、百里香酚(20.02%)。 结论: HD和HS-SPME 2种分析方法共鉴定出22个挥发性成分,主要挥发性物质组分一致。HS-SPME-GC-MS能快速地获得其组成成分,有利于提取易挥发性化合物,特别是对挥发性单萜类和倍半萜类化合物,可应用于阿育魏实挥发性成分的快速分析及质量控制。

Objective: To utilize headspace solid-phase microextraction(HS-SPME) combined with gas chromatography-mass spectrometry technology (GC-MS) as a new method to analyze the volatile components of the fruit of Trachyspermum ammi (L.) Sprague(Uighur traditional medicine). Methods: Four extraction fibers,PDMS,CAR/PDMS,PDMS/DVB and DVB/CAR/PDMS,were chosen for solid phase microextraction to extract the volatile components which were further isolated and identified by GC-MS.The relative mass fraction of each component was determined by the area normalization method.The results from HS-SPME-GC-MS were compared with those obtained from conventional hydrodistillation GC-MS (HD-GC-MS). Results: The volatile components of fruits of T.ammi were effectively extracted by the DVB/CAR/PDMS fiber.The main components were γ-terpinene (26.21%),p-cymene (23.58%) and thymol (20.02%). Conclusions: Twenty-two volatile compounds were identified with HD and HS-SPME,and the main volatile components are consistent.The components can be rapidly obtained by HS-SPME method which is conducive to extract the volatile compounds,specially for monoterpenes and sesquiterpenes compounds.This method is applicable for rapid analysis and quality control of volatile components of Trachyspermum ammi (L.) Sprague fruits.