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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nifdc.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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醋酸泼尼松龙醇质体药物含量及包封率的测定

Determination of content and entrapment efficiency of prednisolone acetate ethosome

分类号:
出版年·卷·期(页码):2013,33 (3):0-0
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:建立醋酸泼尼松龙醇质体含量及包封率的测定方法。 方法:采用注入法制备醇质体,以超滤法分离醇质体和游离药物,建立HPLC测定醋酸泼尼松龙含量的方法。 结果:醋酸泼尼松龙浓度在1.0~50.0 mg·L-1 范围内与峰面积呈良好的线性关系(r=0.9996),回收率为99.8%~100. 8%,日内及日间精密度均小于2%,样品24 h内稳定性良好。超滤法能将醇质体与游离药物良好地分离,超滤回收率为97.2%~97.8%,加样回收率为96.6%~97.5%,平均包封率为(76.79±0.29)%。 结论:该方法准确可靠、方便快捷,可用于醋酸泼尼松龙醇质体中药物含量及包封率的测定。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish a method for the determination of content and entrapment efficiency of prednisolone acetate ethosome. Method: Prednisolone acetate ethosome was prepared with injection method.Ultrafiltration method was employed to separate the free drug from the ethosome.The content of prednisolone acetate was determined by an HPLC method. Results: A good linear relationship was found between the peak area and the concentration of prednisolone acetate ranging from 1.0 mg·L-1 to 50 mg·L-1(r=0.9996).The recovery of prednisolone acetate was in a range of 99.8%-100.8%.Intra-day and inter-day RSDs were less than 2%.Prednisolone acetate was stable in the prepared samples after placing in the auto-sampler for 24 h at ambient temperature.The free drug was well separated from the ethosome by ultrafiltration method.The drug recovery by ultrafiltration was in a range of 97.2%-97.8%,the sample recovery was in a range of 96.6%-97.5%.The average entrapment efficiency of prednisolone acetate in ethosome was(76.79±0.29)%. Conclusion: The method is simple,accurate and suitable for determination of content and entrapment efficiency of prednisolone acetate ethosome.

-----参考文献:---------------------------------------------------------------------------------------
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