RP-HPLC同时测定复方甘草片中4个成分的含量

目的: 采用高效液相色谱法同时测定复方甘草片中吗啡、磷酸可待因、甘草苷和甘草酸的含量。方法: 采用Phenomenex Luna-C₁₈ (250 mm×4.6 mm,5 μm)色谱柱,以乙腈-0.02 mol·L^-1磷酸二氢钾溶液(磷酸调pH至4.0)为流动相,梯度洗脱,流速1.0 mL·min^-1,检测波长为220 nm(0～23 min,检测吗啡和可待因)、254 nm(23～50 min,检测甘草苷和甘草酸),柱温为35 ℃。结果: 4个成分的峰面积与浓度的线性关系良好(r>0.9999);加样回收率为98.0%～100.8%。结论: 该方法可用于复方甘草片中吗啡、磷酸可待因、甘草苷和甘草酸4个成分的含量测定。

Objective: To develop an HPLC method for content determination of morphine, codeine phosphate, liquiritin, glycyrrhizic acid in compound liquorice tablets. Methods: The HPLC analysis was performed on a Phenomenex Luna C₁₈ (250 mm×4.6 mm, 5 μm) column.The mobile phase consisted of acetonitrile and 0.02 mol·L^-1 potassium dihydrogen phosphate (pH was adjusted to 4.0 by phosphoric acid) with a gradient elution at the flow rate of 1.0 mL·min^-1.The detected wavelengths were set separately in different time period (0-23 min, 220 nm for morphine and codeine;23-50 min, 254 nm for liquiritin and glycyrrhizic acid), and the column temperature was 35 ℃. Results: Four active components were separated completely in 50 minutes, and had good linear relationship (r>0.9999) and recoveries(98.0%-100.8%) in their respective detection scopes. Conclusion: The method can be applied for simultaneous determination of four active components (morphine, codeine phosphate, liquiritin, glycyrrhizic acid) in compound liquorice tablets.

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