硫磺熏蒸前后白芍HPLC-UV特征图谱的比较研究

目的: 对白芍硫磺熏蒸前后的HPLC-UV特征图谱进行比较研究,为科学评价及有效控制其质量提供可靠方法。方法: 采用RP-HPLC方法,使用Hypersil ODS C₁₈(250 mm×4.6 mm,5 μm)色谱柱,流动相为乙腈-0.5%醋酸水溶液,流速为1.0 mL·min^-1,二元梯度洗脱,二极管阵列检测器,测定未经硫磺熏蒸和经硫磺熏蒸的白芍各10批,以芍药苷为参照物,检测波长为270 nm。结果: 未经硫磺熏蒸的白芍中共有23个共有峰,经硫磺熏蒸的白芍中共有18个共有峰,通过与对照品的保留时间及紫外光谱比较,5、13、16、17号峰的归属分别为没食子酸、儿茶素、白芍苷、芍药苷。结论: 白芍经硫磺熏蒸后,其成分发生了比较明显的变化,同时白芍在硫磺熏蒸前后,某些共有成分的含量也有不同程度的改变,硫磺熏蒸确实对白芍的质量有非常大的影响。

Objective: To comparatively analyze HPLC-UV specific chromatograms of Paeoniae Radix Alba before and after sulfur-fumigation, so as to provide reliable methods for scientifically evaluating and effectively controlling the quality of Paeoniae Radix Alba. Methods: RP-HPLC method was employed, and the chromatographic conditions were as follows:Hypersil ODS C₁₈ column (250 mm×4.6 mm, 5 μm), binary solvent delivery gradient elution of acetonitrile-water(containing 0.5% acetic acid) as mobile phase with a flow rate of 1.0 mL·min^-1, photodiode array detector.The detective wavelength was set at 270 nm, and ten batches of sun-dried and sulfur-fumigated Paeoniae Radix Alba were respectively analyzed using paeoniflorin as the reference compound. Results: A satisfactory method for HPLC-UV specific chromatograms of Paeoniae Radix Alba before and after sulfur-fumigation was established.Totally 23 common peaks were observed in the specific chromatogram of sun-dried Paeoniae Radix Alba , while only 18 common peaks were found in the specific chromatogram of sulfur-fumigated Paeoniae Radix Alba.The 5th, 13th, 16th and 17th peaks were identified as gallic acid, catechin, albiflorin and paeoniflorin, respectively, by comparisons with the retention times and UV spectra of the reference substances. Conclusion: The constituents in Paeoniae Radix Alba changed remarkably after sulfur-fumigation;moreover, the contents in some common peaks of Paeoniae Radix Alba also changed in different degree before and after sulfur-fumigation.Taken together, sulfur-fumigation makes great impact on the quality of Paeoniae Radix Alba.