RP-HPLC法同时测定爵床药材中6'-羟基-爵床定B、新爵床脂定B和TaiwanE的含量

目的: 建立爵床药材中6'-羟基-爵床定B、新爵床脂定B和Taiwan E 3个活性成分的HPLC含量测定方法。方法: 采用Eclipse XDB-C₁₈色谱柱(4.6 mm×150 mm,5 μm),流动相为乙腈-水,梯度洗脱(0～9 min,40%乙腈;9～10 min,40%→45%乙腈;10～40 min,45%乙腈),流速0.8 mL·min^-1,检测波长256 nm,柱温30 ℃。结果: 6'-羟基-爵床定B、新爵床脂定B和Taiwan E的线性范围分别为0.023～0.454 μg(r=0.9999)、0.022～0.446 μg(r=0.9999)和0.024～0.478 μg(r=0.9998);平均回收率分别为101.7%(RSD=1.6%)、95.7%(RSD=0.30%)和99.4%(RSD=1.7%)。结论: 本方法可用于爵床药材的质量控制。

Objective: To develop a RP-HPLC method for the determination of three active components, 6'-hydroxy-justicidin B, neojusticin B and Taiwan E, in Herba Justiciae. Methods: The sample was analyzed on a Eclipse XDB-C₁₈ column(4.6 mm×150 mm, 5 μm)with a mobile phase consisting of acetonitrile and water(0-9 min, 40% acetonitrile;9-10 min, 40%→45% acetonitrile;10-40 min, 45% acetonitrile)at the flow rate of 0.8 mL·min^-1.The column temperature was set up at 30 ℃, and the detection wavelength was at 256 nm. Results: The linear ranges of 6'-hydroxy-justicidin B, neojusticin B and Taiwan E were 0.023-0.454 μg(r=0.9999), 0.022-0.446 μg(r=0.9999) and 0.024-0.478 μg(r=0.9998), respectively.The average recoveries of the three components were 101.7%(RSD=1.6%), 95.7%(RSD=0.3%) and 99.4%(RSD=1.7%), respectively. Conclusion: The method can be used for the quality control of Herba Justiciae.

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