RRLC-DAD-ESI-MSn分析生脉注射液化学成分
Analysis of chemical constituents of Shengmai injection by RRLC-DAD-ESI-MSn
分类号：
出版年·卷·期（页码）：2013,33 (1):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的: 采用高分离度快速液相色谱(RRLC)-DAD-ESI-MSn联用技术分析生脉注射液中化学成分。方法: 采用Agilent Eclipse Plus C18(2.1 mm×50 mm,1.8 μm)色谱柱,以乙腈-0.1%甲酸为流动相,梯度洗脱,254 nm、正离子模式检测木脂素类成分;203 nm、负离子模式检测皂苷类成分。结果: 利用多级质谱信息,结合对照品对照和文献数据分析,初步确定了注射液中34个成分的结构。结论: RRLC-DAD-ESI-MSn联用技术对于中药注射液物质基础研究具有较好的应用价值。
-----英文摘要：---------------------------------------------------------------------------------------
Objective: To analyze the chemical constituents of Shengmai injection by rapid resolution liquid chromatography (RRLC)-DAD-ESI-MSn. Methods: The chromatographic separation was performed on an Agilent Eclipse Plus C18 column(2.1 mm×50 mm, 1.8 μm), acetonitrile and 0.1% formic acid was used as the mobile phase for gradient separation.Ginsenosides were detected at 203 nm in negative mode, while lignans were detected at 254 nm in positive mode. Results: Based on the MSn information, thirty-four chemical constituents were identified primarily by comparing with reference standards and reference data. Conclusion: RRLC-DAD-ESI-MSn technology is applicable for the study of material base of traditional Chinese medicine injection.
-----参考文献：---------------------------------------------------------------------------------------
1 Drug Specifications Promulgated by the Ministry of Public Health,P R C hina.Part 1,Vol 15(卫生部药品标准一部第十五册).1998.170
2 XIA Jing(夏晶),WANG Ke(王珂),WANG Gang-li(王钢力),et al.Affirming marker peaks for fingerprint chromatography of Shengmai injection(LC-MS/MS法确认生脉注射液指纹图谱的特征峰).Chin Tradit Pat Med(中成药),2007,29(3):313
3 Zheng Chaonan,Hao Haiping,Wang Xuan,et al.Diagnostic fragment-ion-based extension strategy for rapid screening and idention cation of serial components of homologous families contained in traditional Chinese medicine prescription using high-resolution LC-ESI-IT-TOF/MS: Shengmai injection as an example.J Mass Spectrom,2009,44(2):230
4 WANG Zhan-liang(王占良),WANG Hong(王弘),CHEN Shi-zhong(陈世忠).Analysis of ginsenosides in Sheng-Mai-Yin decoction by high performance liquid chromatography-diode array detection-electrospray mass spectrometry(高效液相色谱-二极管阵列检测/质谱法分析生脉饮煎剂中的人参皂苷类成分).Chin J Chromatogr(色谱),2006,24(4):325
5 Huang Xin,Song Fengrui,Liu Zhiqiang,et al.Structural characterization and identification of dibenzocyclootadiene lignans in Fructus Schisandrae using electrospray ionization ion trap multiple-stage tandem mass spectrometry and electrospray ionization fourier transform ion cyclotron resonance multiple-stage tandem mass spectrometry.Anal Chim Acta,2008,615(2):124
6 Shi Peiying,He Qing,Zhang Yufeng,et al.Characterisation and identification of isomeric dibenzocyclooctadiene lignans from Schisandra chinensis by high-performance liquid chromatography combined with electrospray ionisation tandem mass spectrometry.Phytochem Anal,2009,20(3):197
7 Huang Xin,Song Fengrui, Liu Zhiqiang,et al.Studies on lignan constituents from Schisandra chinensis(Turcz.)Baill.fruits using high-performance liquid chromatography/electrospray ionisation multiple-stage tandem mass spectrometry.J Mass Spectrom,2007,42(9):1148
8 LIU Yan(刘燕),NIE Li-xing(聂黎行),CHEN Fang-jun(陈方军),et al.Analysis on chemical constituents of Shenmai injection by RRLC-ion trap mass spectrometry(高分离度快速液相色谱-离子阱质谱分析参麦注射液化学成分).Chin J Pharm Anal(药物分析杂志),2011,31(9):1672
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