β-环糊精在喹诺酮类抗生素药品微生物限度检查方法建立中的应用
Application of β-cyclodextrin in microbial limit test of quinolones antibiotics
分类号:
出版年·卷·期(页码):2013,33 (1):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------
目的: 研究β-环糊精对喹诺酮类抗生素药品抑菌性的中和作用,建立喹诺酮类抗生素药品微生物限度检查方法。方法: 以β-环糊精为中和剂,采用薄膜过滤法对喹诺酮类抗生素药品进行微生物限度方法学研究。结果: β-环糊精对喹诺酮类抗生素药品的抑菌性有较好的中和作用,经过实验,5株验证菌回收率均在70%以上。结论: β-环糊精可作为消除喹诺酮类抗生素药品抑菌活性的中和剂,解决该类药品微生物限度检查方法建立困难问题。
-----英文摘要:---------------------------------------------------------------------------------------
Objective: To study the neutralization effect of β-cyclodextrin against the bacteria restraint of quinolones antibiotics, and establish a microbial limit test method for quinolones antibiotics. Methods: The microbial limit test method of quinolones antibiotics was established using β-cyclodextrin as the neutralizing agent and utilize filtration method. Results: The method can pass the validation test of Chinese Pharmacopoeia (ChP) 2010 version, the rates of recovery of five validation strains were all above 70%. Conclusion: β-Cyclodextrin can neutralize the antiseptic effect of quinolones and the established method can be applied in microbial limit test for quinolones antibiotics preparation control.
-----参考文献:---------------------------------------------------------------------------------------
1 ChP(中国药典).2010.Vol Ⅰ(二部):Appendix(附录)107
2 BP 2002.Appendix XVI B,315
3 EP 5.0.154
4 USP 28.2752
5 JP 14.60
6 LIU Peng(刘鹏),MA Shi-hong(马仕洪),DAI Hui(戴翚),et al.Establishment of method for microbial limit test of gatifloxacin(加替沙星微生物限度检查方法的建立).Chin J Pharm Anal(药物分析杂志),2007,27(6):881
7 LIANG Yue-qiu(梁月秋),LIU Tao(刘涛),ZHU Bin(朱斌),et al.Selection of positive ion used for sterility test of quinolones(应用于喹诺酮类药物无菌检查金属离子的初步筛选).Chin J Pharm Anal(药物分析杂志),2009,29(2):334
8 HU Wen-hong(胡文红),CHENG Chun-lei(程春雷),WANG De-li(王德利).Effect of application of neutralizer on microorganism inspecting method of fluoroquinolone drugs(应用中和剂对氟喹诺酮类药物微生物学检查方法的影响).Food Drug(食品与药物),2011,13(11):417
9 TONG Lin-hui(童林荟).Cyclodextrin Chemistry-Fundamentals and Applications(环糊精化学—基础与应用).Beijing(北京):Science Press(科学出版社),2001
10 ZHANG Qi-ping(张其平),ZHANG Yue-hua(张跃华),WANG Nan-ping(王南平),et al.Study on the inclusion of β-CD and OFLX(氧氟沙星与β-环糊精之间形成包合物的研究).Jiangsu Chem Ind(江苏化工),2005,33(60):47
11 Zhang Min,Li Jinxi,Chao Jianbin,et al.Preparation and spectral investigation of inclusion complex of caffeic acid with hydroxypropyl-cyclodextrin.Spectrochim Acta Part A,2009,71(5):1891
12 Yang Rui,Fu Yan,Li Long-di,et al.Medium effects on fluorescence of ciprofloxacin hydrochloride original research article.Spectrochim Acta Part A,2003,59:2323
13 ZHONG Di-chang(钟地长).Research progress on metal complexes with quinolone antibacterial drugs(喹诺酮类抗菌药物金属配合物的研究进展).J Yichun Univ(Nat Sci)(宜春学院学报自然科学),2007,29(4):77
14 ZHANG Guang-hua(张光华),YU Li(余立).Neutralization of antibacterial activity of drugs in microbial limit test by polysorbate 80 and lecithin(聚山梨酯80和卵磷脂在化学药微生物限度检查时的中和作用).Chin J Pharm Anal(药物分析杂志),2008,28(7):1127
欢迎阅读《药物分析杂志》!您是该文第 1857位读者!