麦冬药材TLC及HPLC特征图谱研究

目的: 建立麦冬药材的TLC及HPLC特征图谱,为科学评价和有效控制其质量提供可靠方法。 方法: 采用3种TLC法建立麦冬薄层特征图谱;采用HPLC法建立麦冬液相特征图谱:色谱柱为Welch AQ C₁₈色谱柱(200 mm×4.6 mm,5 μm),乙腈-水为流动相,梯度洗脱,柱温30 ℃,流速为1.0 mL·min^-1,检测波长205 nm,进样量10 μL。 结果: 麦冬TLC鉴别分离良好;麦冬HPLC特征图谱有6个共有峰,不同来源的川麦冬相似度大于0.94,3批浙麦冬相似度低,HPLC方法的精密度、稳定性和重现性良好。 结论: 所建方法可行,可用于麦冬药材的质量控制。

Objective: To establish the TLC and HPLC characteristic chromatograms of Ophiopogonis Radix,so as to provide a reliable method for scientific evaluation and effective quality control of this medicinal material. Methods: Three kinds of TLC method were adopted to establish the thin-layer characteristic chromatogram of Ophiopogonis Radix.And the HPLC method was used to establish the liquid characteristic chromatogram of Ophiopogonis Radix.The HPLC analysis was performed on a Welch AQ C₁₈column (200 mm×4.6 mm,5 μm).The mobile phase was acetonitrile－water with gradient elution.The flow rate was 1.0 mL·min^-1 and the detection wavelength was 205 nm,the sample volume 10 μL,and the column temperature was 30 ℃. Results: Ophiopogonis Radix was well identified and separated.The HPLC characteristic chromatogram of Ophiopogonis Radix had 6 common peaks.Sichuan Ophiopogonis Radix from different places of origins were of high similarity(>0.94),while the Ophiopogonis Radix of 3 batches were of low similarity.The HPLC method was precise,stable and reproducible. Conclusion: These established methods are practical and suitable for the quality control of Ophiopogonis Radix.

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