HPLC同时测定补肾益寿片中朝藿定A、B、C和淫羊藿苷的含量

目的: 建立高效液相色谱法同时测定补肾益寿片中朝藿定A、朝藿定B、朝藿定C和淫羊藿苷的含量。 方法: 采用Elite Kromasil C₁₈(4.6 mm×250 mm,5 μm)色谱柱,流动相为乙腈(A)-水(B),梯度洗脱(0~24 min,25%A→27%A;24~30 min,27%A→100%A),流速1 mL·min^-1,检测波长270 nm,柱温20 ℃。 结果: 朝藿定A进样量在0.096~0.768 μg,朝藿定B在0.200~1.60 μg,朝藿定C在0.276~2.21 μg,淫羊藿苷在0.416~3.33 μg范围内呈良好线性关系;相关系数分别为0.9991,0.9986,1.0000,0.9997。朝藿定A、朝藿定B、朝藿定C和淫羊藿苷的平均回收率(n=5)分别为97.8%,99.5%,99.2%,100.2%;RSD分别为2.4%,3.9%,3.1%,3.5%。 结论: 本文方法可作为补肾益寿片中多指标成分检测的方法,为补肾益寿片的质量控制提供依据。

Objective: To develop an HPLC method for simultaneous determination of epimedin A,epimedin B,epimedin C and icariin in Bushenyishou tablets. Methods: The Elite Kromasil C₁₈(4.6 mm×250 mm,5 μm) column was adopted with a mobile phase of acetonitrile(A)-water(B) by a gradient eluted program(0-24 min,25%A→27%A;24-30 min,27%A→100%A) at the flow rate of 1.0 mL·min^-1.Detection wavelength was set at 270 nm,and the column temperature was set at 20 ℃. Results: The calibration curves showed a good linearity within the ranges of 0.096-0.768 μg(r=0.9991) for epimedin A,0.200-1.60 μg(r=0.9986) for epimedin B,0.276-2.21 μg(r=1.0000) for epimedin C,and 0.416-3.33 μg(r=0.9997) for icariin.The average recoveries(n=5) were 97.8% for epimedin A,99.5% for epimedin B,99.2% for epimedin C,and 100.2% for icariin,with RSDs of 2.4%,3.9%,3.1% and 3.5%,respectively. Conclusion: The method is suitable for the determination of multi-indexed ingredients and quality control of Bushenyishou tablets.

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