pH、温度和光照等因素对胸腺肽α₁稳定性的影响

目的: 考察pH、温度、光照等因素对胸腺肽α₁稳定性的影响。 方法: 采用HPLC法分析胸腺肽α₁在不同条件下的含量变化,将浓度变化与时间作线性回归,求得药物在不同pH条件下的降解曲线方程,利用降解速率常数反映出胸腺肽α₁在恒温条件下的最适pH。 结果: 酸、碱、光照及高温均能促进胸腺肽α₁的降解,药物的最稳定pH在5~6之间,且在醋酸盐缓冲液中较稳定。 结论: 温度、pH、光照等因素对胸腺肽α₁的稳定性有显著影响。

Objective: To investigate the influence of pH,temperature and light on stability of thymosin α₁. Methods: An adjusted HPLC method was employed to determine concentration changes of thymosin α₁ with these experimental factors mentioned above.The degradation calculations of thymosin α₁ under different pH conditions were obtained by linear regression analysis between concentration and time,whose slopes indicated the sensitivity of the drug stability to these influencing factors. Result: Acid,alkali,light and high temperature could promote the degradation of thymosin α₁.Thymosin α₁ was stable in the acetate buffer,and achieved the highest stability when pH was between 5 and 6. Conclusion: Temperature,pH,light and other factors have a significant effect on the stability of thymosin α₁.

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