柱后衍生阳离子交换色谱法测定桦菌芝中氨基酸的含量

目的: 建立桦菌芝中氨基酸的含量测定方法。 方法: 采用阳离子交换色谱柱分离 17 种氨基酸,通过柱后衍生化检测分析。色谱柱为磺酸基强酸性阳离子交换树脂 LCAK06/Na型(4.6 mm×150 mm),采用线性梯度洗脱,其中A泵(溶液泵)流速为 0.45 mL·min^-1,M泵(茚三酮泵)流速为 0.25 mL·min^-1;检测波长为 440 nm(脯氨酸)和 570 nm (其余氨基酸);进样量为 50 μL。 结果: 该方法中各氨基酸的线性关系良好;精密度 RSD范围0.28%~1.67%,重复性 RSD 范围0.50%~2.13%;平均回收率为98.7%。 结论: 本研究建立的分析方法准确,灵敏度高,具有良好的重复性和回收率,可作为中药材中氨基酸的定量分析和研究方法。

Objective: To establish a determination method for amino acids in Pyropolyporus fomentarius. Methods: A total of 17 amino acids were separated using cation-exchange column,and then analyzed by post-column derivatization.The sulfonic acid cation exchange resin LCAK06/Na type (4.6 mm × 150 mm) column was employed using a linear gradient elution,where solution pump (A pump) was administrated at a flow rate of 0.45 mL·min^-1,ninhydrin pump (M pump) flow rate at 0.25 mL·min^-1; detection wavelength was set at 440 nm(proline) and 570 nm(the other amino acids); injection volume was 50 μL. Results: The method showed a good linear relationship in amino acid determination; the precision RSD was in the range 0.28%-1.67%,the repeatability RSD was in the range of 0.50%-2.13%; the average recovery was 98.7%. Conclusion: The analytical method established is accurate,sensitive,reproducible and feasible,which can be applied to the analysis of amino acids in Chinese crude drug.

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