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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nifdc.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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HPLC同时测定银黄含片中10个活性成分的含量

HPLC simultaneous determination of 10 active constituents in Yinhuang buccal tablets

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出版年·卷·期(页码):2012,32 (10):0-0
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的: 建立HPLC法同时测定银黄含片中10个活性成分(新绿原酸、绿原酸、隐绿原酸、异绿原酸B、异绿原酸A、异绿原酸C、黄芩苷、汉黄芩苷、黄芩素及汉黄芩素)的含量。 方法: 色谱柱:AkzoNobel Kromasil C18柱(250 mm×4.6 mm,5 μm);流动相:乙腈-0.1%磷酸溶液,梯度洗脱;分段变波长测定;柱温:30 ℃;流速:1.0 mL·min-1结果: 10个成分的线性关系良好,精密度、稳定性、重复性的RSD均低于3%,加样回收率为97.3%~99.1%。 结论: 该方法简便、准确、灵敏,可用于银黄含片的质量标准提高研究。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish an HPLC method for the simultaneous determination the contents of 10 active constituents(neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,isochlorogenic acid B,isochlorogenic acid A,isochlorogenic acid C,baicalin,wogonoside,baicalein and wogonin)in Yinhuang buccal tablets. Methods: The chromatographic separation was achieved with a AkzoNobel Kromasil C18 column(250 mm×4.6 mm,5 μm).Acetonitrile-0.1% phosphoric acid aqueous with gradient elution was adopted as the mobile phase,and changeable detection wavelength was applied.The column temperature was 30 ℃ and the flow rate was 1.0 mL·min-1. Results: All the 10 compounds showed good linearity in the range of the tests concentration.The RSD of the precision,reproducibility and stability tests were all less than 3%,the average recoveries were between 97.3%-99.1%. Conclusion: This method is simple,sensitive,and accurate,which can be used for quality control of Yinhuang buccal tablets.

-----参考文献:---------------------------------------------------------------------------------------
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