HPLC同时测定银黄含片中10个活性成分的含量

目的: 建立HPLC法同时测定银黄含片中10个活性成分(新绿原酸、绿原酸、隐绿原酸、异绿原酸B、异绿原酸A、异绿原酸C、黄芩苷、汉黄芩苷、黄芩素及汉黄芩素)的含量。 方法: 色谱柱:AkzoNobel Kromasil C₁₈柱(250 mm×4.6 mm,5 μm);流动相:乙腈-0.1%磷酸溶液,梯度洗脱;分段变波长测定;柱温:30 ℃;流速:1.0 mL·min^-1。 结果: 10个成分的线性关系良好,精密度、稳定性、重复性的RSD均低于3%,加样回收率为97.3%~99.1%。 结论: 该方法简便、准确、灵敏,可用于银黄含片的质量标准提高研究。

Objective: To establish an HPLC method for the simultaneous determination the contents of 10 active constituents(neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,isochlorogenic acid B,isochlorogenic acid A,isochlorogenic acid C,baicalin,wogonoside,baicalein and wogonin)in Yinhuang buccal tablets. Methods: The chromatographic separation was achieved with a AkzoNobel Kromasil C₁₈ column(250 mm×4.6 mm,5 μm).Acetonitrile-0.1% phosphoric acid aqueous with gradient elution was adopted as the mobile phase,and changeable detection wavelength was applied.The column temperature was 30 ℃ and the flow rate was 1.0 mL·min^-1. Results: All the 10 compounds showed good linearity in the range of the tests concentration.The RSD of the precision,reproducibility and stability tests were all less than 3%,the average recoveries were between 97.3%-99.1%. Conclusion: This method is simple,sensitive,and accurate,which can be used for quality control of Yinhuang buccal tablets.

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