不同采收期的紫苏叶和白苏叶中迷迭香酸的含量测定

目的: 测定不同时期采收的紫苏叶和白苏叶中迷迭香酸的含量,确定最佳采收期。 方法: 采用HPLC法测定迷迭香酸的含量。色谱柱:Welch RP-C₁₈柱(250 mm×4.6 mm,5 μm);流动相:甲醇-0.1%磷酸水溶液(39∶61);流速:1.0 mL·min;检测波长:330 nm;柱温:28 ℃。 结果: 双紫苏叶和单紫苏叶中迷迭香酸含量从5~7月逐渐下降,从7~8月逐渐升高并达到最高,8~10月含量又依次下降;白苏叶中迷迭香酸含量从5~8月逐渐升高并在8月达到最高,8~10月含量又依次下降。 结论: 双紫苏叶、单紫苏叶和白苏叶的最佳采收期均为8月。

Objective: To determine the contents of rosmarinic acid so as to find out the best harvest time of Perilla frutescens (L.) Britt.var.acuta (Thunb.) Kudo (abbr:PFA),Perilla frutescens (L.) Britt.var.acuta (Thunb.) Kudo form.discolor (Makino) Kudo (abbr:PFAD) and Perilla frutescens (L.) Britt.var.typica (Makino) Makino (abbr:PFT). Methods: The content of rosmarinic acid was determined on a Welch RP-C₁₈ analytical column(250 mm×4.6 mm,5 μm) with the column temperature at 28 ℃.An isocratic elution of methanol(A) and phosphonic acid solution(B) (39∶61,v/v) was applied.The flow rate was 1.0 mL·min^-1 and the detector wavelength was set at 330 nm. Results: The contents of rosmarinic acid in leaf of PFAD and PFA decreased from May to July,and gradually increased from July to August and reached the highest level in August,and then decreased again from August to October.The contents of rosmarinic acid in leaf of PFT increased from May to August and reached the highest level in August,and then decreased from August to October. Conclusion: August is the best time to harvest leaf of PFA,PFAD and PFT.

1 SUN Xiong-cai(孙雄才).Authentication of Perilla frutescens (L.) Britt.var.acuta (Thunb.) Kudo and Perilla frutescens (L.) Britt.var.typica (Makino) Makino(紫苏和白苏品种的鉴别).Acta Nanjing Pharm Coll(南京药学院学报),1961,6(6):1
2 ChP(中国药典).2010.Vol Ⅰ(一部):318
3 Aritomi M,Kumori T,Kawasaki T.Cyanogenic glycosides in leaves of Perilla frutescens var.acuta.Phytochemistry,1985,24(10):2438
4 LI Wei-guang(李伟光),LIANG Jing-yu(粱敬钰).Research progress of rosmarinic acid(迷迭香酸的研究进展).Strait Pharm J(海峡药学),2004,16(1):1
5 ZHANG Xin(张鑫),ZHANG Zhi-jun(张志军),LI Hui-zhen(李会珍),et al.Extraction and determination of rosmarinic acid from Perilla leaves(紫苏叶中迷迭香酸的提取及测定).Sci Tech Food Int(食品工业科技),2009,30(11):235
6 HUANG Liang-hui(黄亮辉),ZHAO Ying-yong(赵英永),WANG Li-li(王李丽),et al.High-speed counter-current chromatography separation and purification of rosmarinic acid fron Perilla frutescens (L.) Britt.var.acuta (Thunb.) Kudo form.discolor (Makino) Kudo(高速逆流色谱分离纯化紫苏叶中迷迭香酸).Chin J Pharm Anal(药物分析杂志),2011,31(11):2087
7 WANG Yu-ping(王玉萍),ZHU Zhao-yi(朱兆仪),YANG Jun-shan(杨峻山).Quality control for Folium Perilla Ⅰ.Determination of perillaldehyde in Perilla leaf by GC(紫苏叶的质量研究-Ⅰ.气相色谱法测定紫苏叶中紫苏醛的含量).Chin J Pharm Anal(药物分析杂志),2000,20(5):307
8 QIU Qin(邱琴),LING Jian-ya(凌建亚),ZHANG Li(张莉),et al.GC-MS analysis of chemical constituents of the essential oil from Perilla frutescens (L.) Britton. by different extraction methods(不同方法提取的白苏叶挥发油的气质联用成分分析).Chin J Pharm Anal(药物分析杂志),2006,26(1):114
9 JIN Jian-zhong(金建忠).Supercritical CO₂ extraction and analysis of volatile oil of Perilla frutescens leaves(超临界CO₂萃取紫苏叶挥发油及其成分分析).Chin J Pharm Anal(药物分析杂志),2011,31(5):826
10 HUANG Liang-hui(黄亮辉),SU Qi(苏琪),ZHAO Ting-ting(赵婷婷),et al.Determination of rosmarinic acid in seeds and its processed products of Perilla frutescens (L.) Britt.var.typica (Makino) Makino and Perilla frutescens (L.) Britt.var.acuta (Thunb.) Kudo(白苏子和紫苏子生品及其炮制品中迷迭香酸的含量测定).J Chin Med Mater(中药材),2010,33(12):1856