HPLC法测定绞股蓝总苷胶囊中绞股蓝皂苷B的含量
HPLC determination of gypenoside B in Jiaogulan Zonggan capsules
分类号：
出版年·卷·期（页码）：2012,32 (9):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的:建立反相高效液相色谱法测定绞股蓝总苷胶囊中绞股蓝皂苷B的含量。方法:采用Agilent Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5 μm),以乙腈-0.5%磷酸(38:62)为流动相,流速1.0 mL·min-1,检测波长210 nm,柱温30℃。结果:绞股蓝皂苷B进样量在0.04~2.0 μg范围内,与峰面积呈良好线性关系;平均回收率(n=6)为102.1%,RSD为1.2%。结论:该方法简便、准确,重复性好,可作为绞股蓝总苷胶囊的质量控制方法。
-----英文摘要：---------------------------------------------------------------------------------------
Objective:To develop a RP-HPLC method for determination of gypenoside B in Jiaogulan Zonggan capsules. Methods:The separation was performed at 30℃ on an Agilent Eclipse XDB-C18(250 mm×4.6 mm,5 μm)column with a mobile phase of acetonitrile-0.5% phosphoric acid(38:62).The flow rate was 1.0 mL·min-1,and the detection wavelength was 210 nm. Results:The linear range of gypenoside B was 0.04-2.0 μg;The average recovery(n=6)of gypenoside B was 102.1%(RSD=1.2%). Conclusions:The method is simple and accurate with good reproducibility,which can be used for the quality control of Jiaogulan Zonggan capsules.
-----参考文献：---------------------------------------------------------------------------------------
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