UPLC法同时测定黔产隔山消药材中3个C₂₁甾体酯苷的含量

目的:建立同时测定民族药材隔山消中3个C₂₁甾体酯苷(白首乌新苷F、G和H)的UPLC分析法。方法:样品经甲醇超声提取后,用UPLC-PDA进行分析检测;采用Waters Acquity UPLC系统,ACQUITY UPLC BEH C₁₈(2.1 mm×100 mm,1.7 μm)色谱柱,流动相为乙腈-水,梯度洗脱,流速为0.4 mL·min^-1,检测波长220 nm,柱温30℃,进样量2 μL。结果:白首乌新苷F、G和H 3个C₂₁甾体酯苷在检测范围内均呈良好线性(r ≥0.9995);平均加样回收率(n=3)在98.2%~104.1%之间,RSD均不大于1.1%。结论:UPLC分离效果及重复性好,且快速、简便,可作为隔山消药材质量控制的参考方法。

Objective:To develop an ultra performance liquid chromatography(UPLC) method to simultaneously determine three pregnane glycosides(cynanauriculosides F,G,H) in Cynanchum auriculatum . Methods:After the untrasonic-assisted extraction with methanol,the analyses were performed on a Waters UPLC system,equipped with a binary solvent manager,a sampler manager,a column compartment and a PDA detector.An ACQUITY UPLC BEH C₁₈ column(2.1 mm×100 mm,1.7 μm) was used for all analyses.The investigated compounds were separated with the mobile phases of water and acetonitrile in gradient elution,with a flow rate of 0.4 mL·min^-1.The temperature of column was set at 30℃,and the injection volume was 2 μL.The detection wavelength was set at 220 nm. Results:The investigated compounds including cynanauriculoside F,G and H had good linearity(r ≥0.9995) over the tested ranges.The average recoveries(n=3) were 98.2% to 104.1% with RSDs≤1.1%. Conclusions:The developed UPLC method is simple,sensitive and accurate with good repeatability,which is available for quality control of raw material of Cynanchum auriculatum .