α-氰基丙烯酸酯医用胶在大鼠肝脏短期创面局部反应的研究

目的: 研究α-氰基丙烯酸酯医用胶短期肝创面局部反应。 方法: 试验采用SD雄性大鼠肝表面创伤模型,试验组和对照组分别采用在肝表面创伤部位喷涂医用胶和肝表面创伤部位不做处理方法,于术后5 min、1 d、3 d观察、取材,并进行组织学研究。 结果: 大体观察:大鼠皮肤切口愈合良好,肝脏手术区大体正常。病理观察:试验组与对照组在术后1 d均有炎症反应,伴有中性粒细胞、淋巴细胞、吞噬细胞渗出,成纤维细胞增生,无坏死发生,但试验组在3 d时炎症较轻,且创伤修复较好。 结论: 在术后早期,没有发现α-氰基丙烯酸酯医用胶引起局部肝组织坏死,与对照组相比炎症较轻,创伤修复较好。

Objective: To investigate the short-term local reaction of α-cyanoacrylate adhesive on a rat liver wound model. Methods: The SD male rat was applied in this study.Testing medical adhesive was sprayed on the liver trauma surface,while,liver trauma surface of control group did not make any treatments.Rats were sacrificed humanely 5 minutes,1 day and 3 days post-operation.The local reaction was observed macroscopically and microscopically. Results: Gross appearance: There was no abnormal appearance in the operation area.Microscopic appearance: Inflammatory response was observed in both testing and control group after 1 day,accompanied by exudation of neutrophils,lymphocytes and phagocytes,and fibrous cell proliferation; but necrosis was not found.The medical adhesive group displayed minor inflammation and better wound healing than control group after 3 days. Conclusion: α-cyanoacrylate adhesive could not cause local liver tissue necrosis.Only minor inflammation was found.The wound healing of testing group was better than that of control group.

1 Freeny PC,Bush WH Jr,Kidd R.Transcatheter occlusive therapy of genitourinary abnormalities using isobutyl 2-cyanoacrylate (Bucrylate).Am J Roentgenol,1979,133(4):647
2 TIAN Xia(田霞),LU Yong-shun(卢永顺).Sperticular kind of adhesive-α-cyanoacrylate medical adhesive (特种胶粘剂-α-氰基丙烯酸酯系医用胶).New Chem Mater (化工新型材料),2007,35(3):112
3 XIA Hui-sheng(夏穗生), TIAN Xia(田霞),LU Yong-shun(卢永顺).A new generation of spray-type Fuaile medical adhesive(新一代喷涂型福爱乐医用胶).J Clic Surg(临床外科杂志),2003,11(2): 120
4 Braunwald NS,Awe WC.Controlof hemorrhage from the heart and aorta utilizing a plastic adhesive.Surgery,1962,51:786
5 Cameron JL,Woodward SC,Herrmann JB.Pancreatic wounds sealed with plastic adhesive.Arch Surg,1964,89(3):546
6 MENG Xiang-hui(孟祥辉),XU Bai-nan(许百男),WEI Shao-bo(魏少波),et al. The application of Fuaile medical adhesive in the neuro-surgery(福爱乐医用胶在神经外科手术中的应用).J Clin Surg(临床外科杂志),2007,15(9):635
7 ZHANG Tao(张涛),WANG Jian-hua(王建华),WANG Xiao-qiang(王小强).The application of Fuaile medical adhesive in the surgical treatment of colon cancer(福爱乐医用胶在结肠癌手术治疗中的应用).J Clin Surg(临床外科杂志),2004,12(11):716
8 QU Le-feng(曲乐丰),WANG Yu-qi(王玉琦),JING Zai-ping(景在平).Comparesive study between rabbit carotid artery sutureless adhesive anastomosis and conventional suture anastomosis (兔颈动脉无缝线胶黏吻合与传统缝线吻合的对比研究).Chin J Exp Surg (中华实验外科杂志),2004,21(4):411
9 Awe WC,Roberts W,Braunwald NS.Rapidly polymerizing adhesive as a hemostatic agent.Study of tissue response and bacteriological properties.Surgery,1963,54:322
10 Inou T.Studies on the surgical use of plastic adhesive.Am J Proctol,1962,13:219
11 Goetz R H,Weibberg D,Hoppenstein.Vascular necrosis caused by application of methyl 2-cyanoacrylate (eastman 910 monomer):7-month follow up in dogs.Ann Surg, 1966,163(2):242
12 JO JV,Aguilar PD,Yeager GH.Control of Hemorrhage from the liver without the use of sutures or clamps; Preliminary report.Amer Surg, 1962,28:754
13 JO JV,Aguilar PD,Yeager GH.Experimental control of renal hemorrhage by the use of a rapidly polymerizing adhesive.Surgery,1964,55:531
14 Raekallio J,Seligman A M.Acute reaction to arterial adhesive in healing skin wounds.J Surg Res, 1964,4:124
15 Cameron JL,Woodward SC,Pulaski EJ.The degradation of cyanoacrylate tissue adhesive.I.Surgery,1965,58:424
16 Wang YM,Cheng LF,Li N.Histopathological study of vascular changes after intra-arterial and intravenous injection of N-butyl-2-cyanoacrylate.Chin J Dig Dis,2006,7(3):175