RP-HPLC法同时测定小建中颗粒中环磷酸腺苷和甘草酸的含量
RP-HPL Csimultaneous determination of cAMP and glycyrrhizic acid in xiaojianzhong granules
分类号：
出版年·卷·期（页码）：2012,32 (7):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的: 建立高效液相色谱法同时测定小建中颗粒中环磷酸腺苷和甘草酸含量。 方法: 采用Agilent TC-C18(4.6 mm×150 mm,5 μm)色谱柱,流动相为甲醇(A)-0.01 mol·L-1磷酸二氢钾溶液(B),梯度洗脱(0~12 min,90%B;12~30 min,30%B),流速:1.0 mL·min-1,检测波长为250 nm,柱温为30℃。 结果: 环磷酸腺苷和甘草酸在12.5~200 μg·ml-1质量浓度范围内与峰面积呈良好的线性关系,环磷酸腺苷和甘草酸的线性方程分别为:Y=23190X-4.6083(R2=0.9998),Y=11364X+80.342(R2=0.9950),环磷酸腺苷和甘草酸的回收率分别为95.2%和97.5%;RSD分别为2.8%和1.8%。 结论: 本方法操作简便,经方法验证可用于中成药小建中颗粒中环磷酸腺苷和甘草酸的含量测定。
-----英文摘要：---------------------------------------------------------------------------------------
Objective: To simultaneous determine the contents of cAMP and the glycyrrhizic acid in Xiaojianzhong granules by RP-HPLC. Methods: An external standard method by RP-HPLC with an Agilent TC-C18(4.6 mm×150 mm,5 μm)column at the temperature 30℃.The mobile phase consisted of methanol(A)-0.01mol·L-1 KH2PO4(B) with the gradient elution(0-12 min,90%B;12-30 min,30%B),at the flow rate of 1.0 mL · min-1.The absorbance was monitored 250 nm. Results: Linearities of cMAP and glycyrrhizic acid were good in the ranges of 12.5-200 μg·mL-1.The linear equations of cMAP and glycyrrhizic acid were Y=23190X-4.6083(R2=0.9998)and Y=11364X+80.342(R2=0.9950).The average recoveries of cMAP and glycyrrhizic acid were 95.2% and 97.5% with the RSDs 2.8% and 1.8%. Conclusions: The method is simple,believable and can be used for determination of cAMP and glycyrrhizic acid in Chinese patent medicine xiaojianzhong granules.
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1 ChP (中国药典).2010.Vol Ⅰ(一部):508
2 ZHANG Qian(张倩),FAN Ju(樊君),LUO Yun-shu(罗云书).HPLC determination for cAMP in Jujube from Shanbei and Xinjiang(HPLC测定陕北大枣和新疆大枣中环磷酸腺苷含量的研究).Chin J Pharm Anal(药物分析杂志),2008,28(6):895
3 LIU Meng-Jun(刘孟军),WANG Yong-hui(王永蕙).Study on cAMP in branch and leaf of Chinese jujube and seedling of wild jujube(枣枝叶及酸枣幼苗中cAMP的研究).Acta Horticul Sin (园艺学报),1993,20(3):305
4 HE Ping(贺平),JIA Sui-wang(贾随旺),WU Meng-chao(吴孟超),et al.The pharmacokinetics of glycyrrhizin in mice and its binding to human plasma protein (小鼠甘草酸的药代动力学及其与人血浆蛋白结合率).Chin Pharm Bull(中国药理学通报),1998,14(1):89
5 SHEN Yang(沈阳),SHEN Jin-can(沈金灿), ZHUANG Zhi-xia(庄峙厦),et al.Comparison between HPCE and HPLC for the analysis of main active components in licorice(HPCE法与HPLC法用于甘草中主要活性成分甘草酸及其降解产物甘草次酸分析的比较).Chin J Pharm Anal(药物分析杂志),2006,26(4):421
6 XIONG Jian-wen(熊建文),NIE Hui-jun(聂惠君),ZHANG Qiong(张群),et al.Determination of paeoniflorin in Xiaojianzhong granules(小建中颗粒芍药苷的含量测定).J Chin Med Mater(中药材),2003,26(11):817
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