HPLC法测定白木香叶和远志地上部分芒果苷的含量

目的: 建立测定白木香叶和远志地上各部分(包括远志整个地上部分、远志叶和远志茎)芒果苷含量的高效液相色谱法,为寻找富含芒果苷的植物资源奠定基础。 方法: 采用Kromasil 100Â C₁₈(4.6 mm(] 85)为流动相,等度洗脱10 min,流速为1 mL·min^{- 1},检测波长为258 nm,柱温为30℃。 结果: 在本色谱条件下,芒果苷和样品中其他成分分离良好,并且浓度在0.116~580 μg·mL^-1范围内线性良好。加样回收率(n=6)分别为97.5%(白木香叶),97.3%(远志地上部分),97.1%(远志叶),98.5%(远志茎);RSD分别为1.6%,2.1%,1.8%,0.21%。 结论: 本方法操作简单、快捷、准确,重复性好,可用于测定白木香叶、远志地上部分等不同来源植物中芒果苷的含量。

Objective: To develop an HPLC method to determine the content of mangiferin in the leaves of Aquilaria sinensis and in the different aerial parts of Polygala tenuifolia (including the total aerial parts of P. tenuifolia, the leaves of P. tenuifolia and the stems of P. tenuifolia), to acquire some insight for mangiferin-rich herbs. Methods: Chromatographic separation was carried out on a Kromasil 100Â C₁₈(4.6 mm×250 mm, 5 μm) column with an isocratic elution for 10 min at a flow rate of 1.0 mL·min^-1. The mobile phase was composed of acetonitrile and 0.5% H₃PO₄ aqueous solution(15: 85). The detection wavelength was set at 258 nm, and the column temperature was 30℃. Results: Mangiferin was separated well with other components in these herbs with a linear range of 0.116-580 μg·mL^-1. The mean recoveries were 97.5%(the leaves of A. sinensis), 97.3%(the aerial parts of P. tenuifolia), 97.1%(the leaves of P. tenuifolia), 98.5% (the stems of P. tenuifolia), and RSDs were 1.6%, 2.1%, 1.8% and 0.21%, respectively. Conclusion :This method is simple, rapid, repeatable and accurate, and can be used for the quantitation of mangiferin in these herbs.

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