HPLC-DAD法同时测定复合维生素B片中4种维生素的含量
HPLC-DAD simultaneous determination of four vitamins in compound vitamin B tablets
分类号：
出版年·卷·期（页码）：2012,32 (6):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的: 建立反相高效液相色谱法同时测定复合维生素B片中4种维生素含量。 方法: 采用梯度洗脱反相高效液相色谱法,ZORBAX Ecliose XDB-C18(4.6 mm×250 mm,5 μm)色谱柱,流动相A为含0.1%三乙胺的0.05 mol·L-1磷酸二氢钾溶液(pH 4.6);流动相B为甲醇,检测波长为266 nm、柱温为40 ℃、流速为1.0 mL·min-1。 结果: 4种维生素含量测定方法的线性关系良好,r 0.999 5~0.999 9,方法回收率均在99.2%~100.6% 范围内,RSD均在0.8%~2.0% 之间。 结论: 本方法简便快捷,灵敏准确,可以用于复合维生素B片的质量控制。
-----英文摘要：---------------------------------------------------------------------------------------
Objective:To establish a method for determination of four vitamins in Compound vitamin B tables.Methods:RP-HPLC was adaptded on a column of ZORBAX Eclipse XDB-C18(4.6 mm×250 mm,5 μm),gadient elution with the mobile phase of A:0.05 mol·L-1 potassium dihydrogen phosphate(pH 4.6 adjusted with 0.1% triethylamine,)and B:methanol at a flow rate of 1.0 mL·min-1 and the detection wavelength was 266 nm with the column temperatureat was 40 ℃.Results:In the linear range the correlation coefficients ranged from 0.9995-0.9999.The average recoveries were 98.4%-100.4% with corresponding RSD of 0.6%-2.0%.Conclution:This method is simple,rapid,sensitive and accurate.It is available to the quality control of compound vitamin B tablets.
-----参考文献：---------------------------------------------------------------------------------------
1 LU Guo-rong(陆帼蓉),WANG Gui-zhen(王桂珍).Determination of vitamin B1、B2、B6 and nicotinamide in compound vitamin B tablets by RP-HPLC(RP-HPLC 法测定复合维生素B片中维生素B1、B2、B6和烟酰胺的含量).J China Pharm Univ(中国药科大学学报),1995,,2(3):177
2 WU Fang(吴芳),XU Hong-yan(徐红艳).Contents determination of the four components in compound vitamin B tablets by HPLC(HPLC法测定复合维生素B 片中四种成分的含量).China Med Herald(医药导报).2007,4(31):95
3 CHEN Guang-long(陈光龙),LI Yu-lan(李玉兰),LIU Min(刘敏),et al.Determination of four vitamins in compound vitamin B tablets by HPLC(高效液相色谱法测定复合维生素B片中4种组分的含量.China Hosp Pharm J(中国医院药学杂志),2006,26(10):1256
4 YIN Jang-wei(尹江伟),SU Hong-wei(苏红卫).HPLC determination of water-soluble Vitamins(高效液相色谱法同时测定水溶性维生素).J Luzhou Med Coll(泸州医学院学报).2000,23(5):425
5 JIANG Ye(蒋晔),LIU Hong-ju(刘红菊),HAO Xiao-hua(郝晓花).RP-HPLC determination of nine water-soluble vitamins(反相高效液相色谱法同时测定9种水溶性维生素).Chin J Pharm Anal(药物分析杂志).2005,,25(3):339
6 ChP(中国药典).2010.Vol Ⅱ:896
7 Tavcar-Kalcher G,Vengust A.Stability of vitamins in premixes.Anim Feed Sci Technol,2007,132(1):148
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