HPLC法和氨基酸分析仪(AAA)法测定肠外营养注射液(25)中18种氨基酸的含量的比较

目的: RP-HPLC法和氨基酸分析仪(amino acid analyzer,AAA)法测定氨基酸含量的比较。 方法: 采用高效液相色谱仪和氨基酸分析仪测定肠外营养注射液(25)中18种氨基酸的含量。采用C₁₈柱和异硫氰酸苯酯进行柱前衍生化,加入二硫代二丙酸作为保护剂,同时测定半胱氨酸;AAA法采用离子交换色谱柱和茚三酮柱后衍生化进行,不需加入保护剂,直接测定半胱氨酸。 结果: RP-HPLC法和AAA法的重复性分别小于1.1%和3.1%,2种测定方法的相对偏差在0.19%~8.2%之间。 结论: AAA法更适于肠外营养注射液(25)种18中氨基酸的测定。

Objective: To compare the RP-HPLC and amino acid analyzer(AAA) methods for determining concentrations of amino acids. Method: Eighteen kinds of amino acids in total parenteral nutrition injection(25) were measured by an HPLC and amino acid analyzer.One C₁₈ column was used for HPLC method with phenyl isothiocyanate precolumn derivation,dithio-dipropanoic acid was added as protective agent and cysteine was determined in the meanwhile;an ion exchange chromatography column was used for AAA method with ninhydrin postcolumn derivation,no protective agent was used and cysteine was determined directly. Result: The repeatability of RP-HPLC and AAA methods were less than 1.1% and 3.1%,respectively.The standard deviation of two methods ranged from 0.19%-8.2%. Conclusion: AAA method is more suitable for determining the concentrations of amino acids than HPLC method.

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