生物分配胶束色谱法预测中药制剂消炎利胆片膜通透性的研究

目的: 基于生物分配胶束色谱法进行复杂中药制剂消炎利胆片的分离和膜通透性预测。 方法: 考察了生物分配胶束色谱的保留因子与药物膜通透性的相关性,以非离子型表面活性剂Brij 35作为流动相,模拟生物体内环境,得到各受试药物的保留因子Log k,与膜通透性参数(口服吸收率、Log P_app、Log BB)数据进行相关分析得出定量保留-膜通透性(QRPR)模型。 结果: 预测了消炎利胆片10个主要保留成分的透膜吸收情况。 结论: 生物分配胶束色谱可以用于中药复杂成分的分离,以及预测药物主成分的透膜吸收活性。

Objective: To separate the complex ingredients in traditional Chinese medicine (TCM) Xiaoyanlidan tablets and estimate the permeability by biopartitioning micellar chromatography (BMC). Methods: The correlation between retention factors in BMC and drug permeability was investigated.Non-ionic Brij35 was used as mobile phase to mimic the in vivo environment,and different concentrations of Brij-35 were studied.The logarithm of capacity factor (Log k) and permeability parameter data (date of oral absorption,Log P_app,Log BB) of test drugs were fitted to model the quantitative retention-permeability relationship (QRPR) models. Results: The permeability of the ten retained components in Xiaoyanlidan tablets were calculated and discussed. Conclusion: The results indicate that BMC can separate the complex components in TCM,as well as predict the membrane absorption activity.