RP-HPLC法测定莽吉柿果皮中3个氧杂蒽酮类化合物的含量

目的: 建立一种莽吉柿果皮中3个氧杂蒽酮类化合物的RP-HPLC含量测定方法。 方法: 色谱柱为Waters XTerra RP18色谱柱(150 mm×4.6 mm,5 μm),以水-0.5%醋酸(A)、甲醇-0.5%乙酸(B)为流动相,梯度洗脱,流速为1.0 mL·min^-1,检测波长为317 nm,柱温为30 ℃。 结果: 3-异倒捻子素、α-倒捻子素、β-倒捻子素浓度分别在21.3~213,107.7~1077,10.6~106 μg·mL^-1范围内线性关系良好(r ≥ 0.9991);平均回收率分别为 102.6%,98.5%,103.5%,RSD 分别为1.7%,1.2%,2.0%(n=6)。 结论: 所建立的方法便捷、准确,重复性好,可以为莽吉柿果皮的质量控制提供一定的参考。

Objective: To establish an HPLC method of the determination of 3 xanthones content in mangosteen fruit skin. Methods: The content of 3 xanthones were determined by Waters XTerra RP18(150 mm×4.6 mm,5 μm) column,the mobile phase consisted of water-0.5% acetic acid(A) and methanol-0.5% acetic acid(B) with gradient elution at the flow rate of 1.0 mL·min^-1,the detection wavelength was 317 nm,and the temperature of column was 30 ℃. Results: The calibration curves of 3-isomangostin,α-mangostin,β-mangostin were linear in the ranges of 21.3-213,107.7-1077,10.6-106 μg·mL^-1(r ≥ 0.9991),respectively.The average recoveries of 3 xanthones were 102.6%,98.5%,103.5% and RSDs(n=6) were 1.7%,1.2%,2.0%,respectively. Conclusion: The method is easy,sensitive,specific,and accurate with good reproducibility.It can be used for quality control of mangosteen fruit skin.

1 SUN Jun-xiu(孙俊秀).Queen of tropical fruits:mangosteen(果中皇后山竹).J Sichuan High Inst Cuisine(四川烹饪高等专科学校学报),2004,(1):18
2 Moongkarndi P,Kosem N,Kaslungka S,et al.Antiproliferation,antioxidation and induction of apoptosis by Garcinia mangostana(mangosteen) on SKBR3 human breast cancer cell line.J Ethnopharmacol,2004,90(1):161
3 Suksamrarn S,Suwannapoch N,Phakhodee W,et al.Antimycobacterial activity of prenylated xanthones from the fruits-Garcinia mangostana.Chem Pharm Bull(Tokyo),2003,51(7):857
4 Sakagami Y,Iinuma M,Piyasena KG,et al.Antibacterial activity of alpha-mangostin against vancomycin resistant Enterococc(VRE) and synergism with antibiotics.Phytomedicine,2005,12(3):203
5 Nabandith V,Suzui M,Morioka T,et al.Inhibitory effects of crude apha-mangostin,a xanthone derivative,on two different categories of colon preneoplastie lesions induced by l,2-dimethylhydrazine in the rat.Asian Pac J Cancer Prey,2004,5(4):433.
6 Suksamrarn S,Komutiban O,Ratananukul P,et al.Cytotoxic prenylated xanthones from the young fruit of Garcinia mangostanal.Chem Pharm Bull(Tokyo),2006,54(3):301
7 Mahabusarakam W,Kuaha K,Wilairat P,et al.Prenylated xanthones as potential antiplasmodial substances.Planta Med,2006,72(10):912
8 ZHAO Yan(赵岩),LIU Jin-ping(刘金平),LU Dan(卢丹),et al.Determination of alpha-mangostin in Pericarpium Garciniae mangostanae L.by RP-HPLC-UVD(RP-HPLC-UVD法测定莽吉柿中α-倒捻子素的含量).J Anhui Agri Sci(安徽农业科学),2010,38(11):5626
9 FENG Jian-guang(冯建光),CHEN Li-qiong(陈利琼).Determination of α-mangostin content in mangosteen fruit skin and mangosteen fruit skin extract(山竹壳中α-倒捻子素的测定方法).J Food Sci Biol(食品与生物技术学报),2009,28(2):256