UPLC-QTOF-MS结合主成分分析法用于龟甲胶、鹿角胶中添加牛皮源成分的检测研究

目的: 建立龟甲胶、鹿角胶中非法掺入牛皮源成分的检测方法。 方法: 采用胰蛋白酶对龟甲胶、鹿角胶、牛皮源成分进行酶解,利用超高效液相色谱-四极杆飞行时间质谱(UPLC-QTOF-MS)进行测定,采用Markerlynx软件对3种胶类的液质数据进行主成分分析,找出鉴别牛皮源成分的专属性特征肽段,并对特征肽的序列进行确认。 结果: 对19批样品进行检测发现,其中8批龟甲胶及8批鹿角胶中可检出牛皮源成分的特征肽段。 结论: 所建立的方法经验证,专属性较强,可用于龟甲胶、鹿角胶中非法掺入牛皮源成分的检测。

Objective: To develop an ultra performance liquid chromatography/time of flight mass spectrometry(UPLC-QTOF) method for identification of bovine-hide gelatin from glue of tortoise shell and deer-horn gelatin. Methods: The sample was analyzed on an UPLC coupled with QTOF mass spectrometry after digested by trypsin.The UPLC-MS data of trypsin digested samples were subjected to principal component analysis (PCA) in order to identify bovine-hide gelatin from glue of Tortoise shell and deer-horn gelatin,and the sequence of marker peptides given by loadings plot of PCA were identified based on the comparison of recorded LC-MS data with the previously reported database of the corresponding gelatin variants by the Biopharmalynx 1.2. Results: The marker peptides of bovine-hide gelatin can be detected from 16 out of 19 samples of the glue of tortoise shell and deer-horn gelatin bought in the market. Conclusion: The results of this study indicate that the proposed method is reliable for identification of bovine-hide gelatin from glue of tortoise shell and deer-horn gelatin.