HPLC法测定钻山风糖浆中芒柄花素的含量

目的: 采用反相高效液相色谱法测定钻山风糖浆中芒柄花素的含量。 方法: 采用Diamonsil C₁₈(4.6 mm×250 mm,5 μm)色谱柱,以乙腈-0.5%磷酸(35: 65)为流动相,流速1.0 mL·min^-1,检测波长248 nm,柱温30 ℃。 结果: 芒柄花素进样浓度在1.505~75.25 μg·mL^-1范围内,与峰面积呈良好线性关系;平均回收率(n=6)为98.6%,RSD为1.7%。 结论: 该方法简便、准确,重复性好,可作为钻山风糖浆质量控制方法。

Objective: To develop an RP-HPLC method for the determination of formononetin in Zuanshanfeng syrup. Methods: The separation was performed at 30 ℃ on a Diamonsil C₁₈(4.6 mm×250 mm, 5 μm) column with a mobile phase of acetonitrile-water containing 0.5% phosphoric acid(35: 65). The flow rate was 1.0 mL·min^-1, and the detecting wavelength was 248 nm. Results: The linear range of formononetin was from 1.505 to 75.25 μg·mL^-1, the average recovery(n=6) of formononetin was 98.6%(RSD=1.7%). Conclusion: The method is simple, accurate and repeatable, which can be applied to the quality control of Zuanshanfeng syrup.

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