不同产地、不同采收期黄芪药材及饮片中毛蕊异黄酮葡萄糖苷及芒柄花素含量测定

目的: 采用HPLC法测定不同产地、不同采收期黄芪药材及饮片中毛蕊异黄酮葡萄糖苷及芒柄花素含量。 方法: 采用Agilent ZORBAX Eclipse XDB-C₁₈(250 mm×4.6 mm,5 μm)色谱柱,流动相为乙腈-水,梯度洗脱,流速1 mL·min^{- 1},柱温35 ℃,检测波长260 nm。 结果: 毛蕊异黄酮葡萄糖苷、芒柄花素线性范围分别为0.1272~12.72 μg(r=0.9999)和0.003344~2.508 μg(r=0.9999),平均回收率(n=6)分别为98.9%(RSD=1.7%)和99.0%(RSD=2.3%)。不同产地黄芪药材及饮片中毛蕊异黄酮葡萄糖苷和芒柄花素含量差异较大,生长年限6年的黄芪中毛蕊异黄酮葡萄糖苷和芒柄花素含量最高。 结论: 该方法简单快捷,适合于黄芪中黄酮类化合物的含量测定研究,为寻找更佳黄芪产地及黄芪采收期提供了依据。

Objective: To establish an HPLC method for the determination of calycosin-7-O-β-D-glucoside and formononetin in crude drugs and slices of Astragalus from different habitat and gathering periods. Methods: ZORBAX Eclipse XDB-C₁₈(250 mm×4.6 mm, 5 μm ) was adoped with a mobile phase of acetonitrile-water, the separation was carried out with a gradient program at a flow rate of 1 mL·min^-1, column temperature was 35 ℃ and detection wavelength was 260 nm. Results: Calycosin-7-O-β-D-glucoside and formononetin showed good linearity in the ranges of 0.1272-12.72 μg(r=0.9999) and 0.003344-2.508 μg (r=0.9999) with average recoveries of 98.9% and 99.0%, respectively.The contents of calycosin-7-O-β-D-glucoside and formononetin in crude drugs and slices of Astragalus from different habitat marked difference, and the contents of calycosin-7-O-β-D-glucoside and formononetin grown for six years was the highest. Conclusion: The method is simple, rapid for determination the contents of calycosin-7-O-β-D-glucoside and formononetin in Astragali Radix, and can be used as a basis for finding better habitat and gathering periods of Astragali Radix.

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