高效液相色谱法测定王不留行中王不留行环肽A和王不留行环肽B

目的: 建立HPLC法测定王不留行中王不留行环肽A和王不留行环肽B含量。 方法: 采用超声辅助提取王不留行环肽A和王不留行环肽B, 选用Agilent C₁₈色谱柱(250 mm×4.6 mm,5 μm),以乙腈-水为流动相进行梯度洗脱,流速为1.0 mL·min^-1,检测波长为220 nm。 结果: 王不留行环肽A和王不留行环肽B进样浓度分别在2.57~12.85 μg·mL^-1和0.95~13.54 μg·mL^-1范围内线性关系良好(r≥0.9996);平均加样回收率(n=6)分别为97.1%和95.9%,RSD分别为1.3%和1.7%。 结论: 该方法简便、准确,重复性好,可用于王不留行中王不留行环肽A和王不留行环肽B的含量测定。

Objective: To establish an HPLC method for the determination of segetalin A and segetalin B in Vaccariae Semen. Methods: Analysis was carried out on an Agilent C₁₈(250 mm×4.6 mm, 5 μm) column with acetonitrile-water as mobile phase in gradient elution. The flow rate was 1.0 mL·min^-1 and detective wavelength was 220 nm. Results: The linear ranges of segetalin A and segetalin B were 2.57-12.85 μg·mL^-1 and 0.95-13.54 μg·mL^-1 (r≥0.9996),respectively. The average recoveries were 97.1% and 95.9% with RSDs of 1.3% and 1.7%,respectively. Conclusion: The results show that the method is simple, accurate and repeatable, and can be used for the quantitative analysis of segetalin A and segetalin B in Vaccariae Semen.

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