土壤—温郁金体系中硒的形态分析

目的: 研究土壤-温郁金体系内硒(Se)的形态分布及其温郁金对土壤中硒的吸收富集情况,旨在为温郁金药材的进一步开发研究提供理论和实验依据。 方法: 采用盆栽方法,在温郁金种植的土壤中施以不同浓度的硒,用连续浸提法提取各形态硒,通过氢化物发生—原子吸收法光谱法检测硒的含量。 结果: 根系土壤中,可利用态硒含量所占比值最高,易于硒的迁移,当土壤中硒浓度低时,温郁金块根和叶子中有机硒含量大于无机硒,且以蛋白硒为主,而当土壤中硒浓度增加到0.5 mg·kg^-1时,对温郁金产生毒害作用,块根中各有机态硒的含量呈现下降趋势。 结论: 温郁金药材中硒含量与其在土壤中含量呈较强的正相关性(P<0.05)。

Objective: In order to provide theoretical and experimental guidance for the further exploitation of Curcuma wenyujin,the speciation of selenium(Se) in Soil-Curcuma wenyujin System and the Curcuma's ability of absorbing and accumulating selenium from soil were researched. Methods: By introducing potted plant and inflicting different Se concentration in Curcuma wenyujin soil,the all species of selenium was extracted using the sequential extraction method and the content of species of Se was determined by hydride generation-atomic absorption spectrometry(HG-AAS). Results: In Curcuma wenyujin soil,the proportion of bio-available form of Se was highest and made Se easily migrate.With the low concentration of Se in soil,the organic selenium content was higher than inorganic in roots and leaves of Curcuma wenyujin,in which the selenium in protein was the main form.However,when the concentration of selenium reached to 0.5 mg·kg^-1,the Curcuma wenyujin was poisoned and all the organic selenium content in Curcuma wenyujin roots showed downtrend. Conclusion: There was a positive correlation of selenium content in Curcuma wenyujin and soil(P<0.05).

[1] Longnecker MP,Taylor PR,Levander OA,et al.Selenium in diet,blood,and toenails in relation to human health in a seleniferous area.Am J Clin Nutri,1991,53:1288
[2] Rayman MP.Selenium in cancer prevention:a review of the evidence and mechanism of action.Proc Nutr Soc,2005,64(4):527
[3] Pitts L,Worsfold PJ,Hill SJ.Selenium speciation a flow injection approach employing on-line microwave reduction followed by hydride generation quartz furnace atomic absorption spectrometry.Analyst (Cambridge,UK),1994,119:2785
[4] Calle-Guntinas MB,Ceulemans M,Witte C,et al.Evaluation of a purge-and-trap injection system for capillary gas chromatography-microwave induced plasma-atomic emission spectrometry for the determination of volatile selenium compounds in water.Mikrochim Acta,1995,120:73
[5] ChP(中国药典).2005.Vol Ⅰ(一部):144
[6] WANG Xue-bao(王学宝),X IANG Zheng(向铮),LIN Dan(林丹),et al.Analysis and determination of
[11] elements in Curcuma wenyujin collected at different developmental periods(不同生长时期温莪术11种金属元素含量测定与分析).Chin J Pharm Anal(药物分析杂志),2010,30(8):1462
[7] Chao TT,Sanzolone RF.Fractionation of soil selenium by sequential partial dissolution.Soil Sci Soc Am J,1989,53,:385
[8] FANG Jin-mei(方金梅).Selenium speciation analysis and its transformation and enrichment in soils of Fuzhou city(福州市土壤硒形态分析及其迁移富集规律).Rock Mineral Anal(岩矿测试),2008,27(2):103
[9] Manning BA,Burau RG.Selenium immobilization in evaporation pond sediments by in situ precipitation of ferric oxyhydroxide.Environ Sci Technol,1995,29:2639
[10] Gustafssow JP,Johnsson L.Selenium retention in the organic matter of Swedish forest soils.Eur J Soil Sci,1992,43 (3):461
[11] LI Li-ping(李莉萍),WANG Jun(王军).Advances on existing form of and analysis method for selenium in soil-plant system(土壤-植物系统中硒的赋存形态及其分析方法研究进展).Chin J Trop Agr(热带农业科学),2009,29(2):58
[12] Marchiol L,Assolari S,Sacco P,et al.Phytoextraction of heavy metals by canola (Brassica napus) and radish (Raphanus sativus) grown on ulticontaminated soil.Environ Pollut,2004,132:21