胶束薄层色谱分离甘草活性成分的影响因素及优化方法

目的: 为甘草的胶束薄层色谱指纹图谱寻找最优胶束流动相。 方法: 首先采用单因子法,寻找影响甘草胶束薄层色谱的影响因素,在此基础上,采用控制加权可变步长单纯形优化法进行甘草胶束薄层色谱指纹图谱的流动相优化。 结果: 对甘草的胶束薄层色谱分离条件(表面活性剂的种类和含量、醇和酸改性剂的影响等)进行了实验,表明纯胶束薄层色谱的柱效较低,加入醇和酸类改性剂后柱效有明显提高。通过对改性胶束的进一步优化(控制加权可变步长单纯形优化法),得到优化的甘草改性胶束展开剂组成为:0.23 mol·L^-1的SDS＋16%(v/v)正丁醇＋11%(v/v)甲酸。本研究对胶束薄层色谱的一些分离机理亦进行了探讨。 结论: 胶束薄层色谱的表面活性剂和各添加剂间存在交互作用,需采用合适的优化方法,才能达到分离中药材复杂活性成分群的目的。

Objective: To seek the best mobile phase of micellar thin-layer chromatography(MTLC)of licorice fingerprint. Method: Single impact factor of mobile phase in licorice MTLC fingerprint was tested individually and controlled weighted centroid simplex method was used finally to optimize the mobile phase of licorice MTLC fingerprint. Results: The selectivity of micellar TLC was studied for the separation of active components in licorice under various operation variables including concentrations of surfactant and co-surfactant,surfactant type,addition of acid modifier,et al.The results show that the low chromatography efficiency of micellar TLC could be improved by the addition of alcohol and acid modifier.Using controlled weighted centroid simplex method,optimization micellar TLC developing system of licorice was achieved which consists of 0.23 mol·L^-1 SDS,16%(v/v)n-butanol and 11%(v/v)methyl acid.In addition,the separation mechanism of micellar TLC was also discussed. Conclusion: he appropriate optimization method should be tried to get the reliable mobile phase for the alternate impact of several parameters in MTLC.