亲水作用色谱－蒸发光散射法测定益母草中盐酸水苏碱的含量

目的: 建立亲水作用色谱-蒸发光散射检测器联用测定益母草中盐酸水苏碱含量的方法。 方法: 采用HILIC 柱(250 mm×4.6 mm,5 μm);乙腈-0.2%冰醋酸溶液(80:20)为流动相;Alltech2000蒸发光散射检测器:漂移管温度80℃,气体流速2.0 L·min^-1;流速0.5 mL·min^-1。 结果: 盐酸水苏碱进样量在0.536~10.713 μg范围内呈良好的线性关系,平均回收率为101.9%,RSD为1.4%。 结论: 此方法简便、灵敏度高,重现性好,可用于益母草的含量测定。

Objective: To establish a HILIC-ELSD method for determination of Stachydrine hydrochloride in Herba Leonuri. Method: The separation was performed on a HILIC column (250 mm ×4.6 mm,5 μm)with acetonitrile-0.2% glacial acetic acid solution (80:20)as mobile phase at a flow rate of 0.5 mL·min^-1.An Alltech 2000 evaporative light-scattering detector (ESLD)was used as detector with gas flow rate of 2.0 L·min^-1 and drift tube temperature of 80℃. Results: There was a good linear relationship with the range of 0.536–10.713 μg.The average recovery was 101.9% (RSD = 1.4%). Conclusion: The method is simple,sensitive and reliable,it can be used as quantitative analysis method for Herba Leonuri.

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