毛细管区带电泳法分离盐酸美沙酮对映体

目的: 采用毛细管区带电泳法对盐酸美沙酮对映体进行了拆分。 方法: 比较了3种衍生化β-环糊精为手性添加剂的分离效果,对缓冲液的pH及浓度、手性添加剂的浓度、柱温、分离电压等方面进行了考察及优化。 结果: 确定采用75 μm×75 cm未凃渍石英玻璃管柱,运行缓冲液为含50 mmol·L^-1的Tris和10 mmol·L^-1的HP-β-环糊精的水溶液(以磷酸调节pH至2.0),分离电压为25 kV,柱温25 ℃,检测波长为205 nm, 结论: 该方法简便、快速,在15 min内分离度可达到1.9。

Objective: To establish a CE method for the separation of the enantiomers of methadone hydrochloride. Methods: Using 3 β-cyclodextrin derivatives as chiral selector,the conditions for the enantiomeric separation were optimized,which including the concentration of the chiral selector,the pH of the buffer,the concentration of the buffer,column temperature,voltage. Results: The separation was performed on a fused-silica tubing 75 μm×75 cm at 25 ℃,with 50 mmol·L^-1 Tris-10 mmol·L^-1的HP-β-CD buffer (adjust pH to 2.0 with phosphoric acid)as the mobile phrase.Samples were separated at 25 kV.The detection wavelength was set at 205 nm. Conclusion: It is a simple,rapid method,which the resolution of the enantiomers can up to 1.9 within 15 min.

[1] FENG Xian-xiang(冯献湘),WEI Hong-wei(韦红卫).Progress in study on methadone in maintenance treatment of heroin addicts(美沙酮治疗海洛因成瘾者研究进展).Med Recap (医学综述),2008,14(5):745
[2] Foster DJR,Somogyi AA,White JM,et al.Population pharmacokinetics of (R)-,(S)- and racmethadone in methadone maintenance patients.Br J Clin Pharmacol,2004,57(6):742
[3] Hutchinson MR,Somogyi AA.(S)-(+)-methadone is more immunosuppressive than the potent analgesic (R)-(-)-methadone.Inter Immunopharmacol,2004,(4):1525
[4] Pham-Huy C,Chikhi-Chorfi N,Galons H,et al.Enantioselective high-performance liquid chromatography determination of methadone enantiomers and its major metabolite in human biological fluids using a new derivatized cyclodextrinbonded phase.J Chromatogr B,1997,700(1-2):155
[5] Rudaz S,Venthey JL.Chiral stationary phases in HPLC for the stereoselective determination of methadone.Chirality,1999(11):319
[6] WANG Yuan–chao(王园朝),JIN Ying-zhi(金英芝),LUO Jin-wei(罗经文).Enantionseparation of drugs by capillary electrophoresis and their separation mechanism(10种手性药物对映体毛细管电泳拆分方法及机理探讨).J Anal Sci (分析科学学报),2009,29(1):21
[7] Deeb SE,Hasemann P,Watzig H.Strategies in method development to quantify enantiomeric impurities using CE.Electrophoresis,2008,29:3552
[8] LI guan-bin,LIN xiu-li,ZHU chen-fu,et al.New derivative of β-cyclodextrin as chiral selectors for the capillary electrophoretic separation of chiral drugs.Anal Chim Acta,2000,421 (1):27
[9] WeiI SL,Song GQ,Lin JM.Separation and determination of norepinephrine,epinephrine and isoprinaline enantiomers by capillary electrophoresis in pharmaceutical formulation and human serum. J Chromatogr A,2005 1098 (1-2):166