RP-HPLC法同时测定保济丸中厚朴酚、和厚朴酚、木香烃内酯及去氢木香内酯的含量

目的: 建立RP-HPLC法同时测定保济丸中厚朴酚、和厚朴酚、木香烃内酯及去氢木香内酯的含量。 方法: 采用Agilent Extent-C₁₈(250 mm× 4.6 mm,5 μm)色谱柱,以乙腈-水(47:53)为流动相,流速为1.0 mL·min^-1,柱温为35 ℃,检测波长为225 nm。 结果: 厚朴酚、和厚朴酚、木香烃内酯及去氢木香内酯的浓度分别在2.744~137.2 μg·mL^-1(r=0.9999),2.836~141.8 μg·mL^-1(r=0.9999),3.164~158.2 μg·mL^-1(r=0.9998)及3.148~157.4 μg·mL^-1(r=0.9999)范围内与峰面积线性关系良好;方法回收率(n=3)为96.3%~102.7%,RSD为0.2%~1.7%。 结论: 本方法简便、准确,重复性好,可用于同时测定保济丸中厚朴酚、和厚朴酚、木香烃内酯及去氢木香内酯的含量。

Objective: To develop an RP-HPLC method for the simultaneous determination of magnolol,honokiol,costunolide and dehydrocostuslactone in Baoji pills. Methods: The analysis was carried out on an Agilent Extent-C₁₈(250 mm×4.6 mm,5 μm)column;The mobile phase was acetonitrile-water(47:53)and the flow rate was 1.0 mL·min^-1;The column temperature was 35 ℃ and the detection wavelength was set at 225 nm. Results: The linear ranges of magnolol,honokiol,costunolide and dehydrocostuslactone were 2.744-137.2 μg·mL^-1(r=0.9999),2.836-141.8 μg·mL^-1(r=0.9999),3.164-158.2 μg·mL^-1(r=0.9998)and 3.148-157.4 μg·mL^-1(r=0.9999),respectively.The average recovery(n=3)was 96.3%-102.7% and RSD was 0.1%-1.7%. Conclusion: The method is simple,and can be used for determination of the four components in Baoji Pills with satisfactory accuracy and repeatability.

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