熊胆丸定性定量方法的研究

目的: 建立熊胆丸的定性定量方法。 方法: 采用TLC法对方中的熊胆、黄连、龙胆、栀子进行定性鉴别;采用GC法同时测定熊胆丸中冰片、薄荷脑的含量。采用安捷伦DB-WAX毛细管柱(30 m×0.45 mm×0.85 μm),载气为氮气,FID检测器,流速:3.0 mL·min^-1;分流比为25∶ 1;进样体积为1 μL。 结果: TLC色谱中可检出熊胆、黄连、龙胆、栀子;龙脑浓度在56.0～672.0 μg·mL^-1范围内呈线性关系,3个浓度的平均回收率(n=3)分别为99.4%,101.6%,100.7%;薄荷脑浓度在56.9～683.3 μg·mL^-1内呈线性关系,3个浓度的平均回收率(n=3)分别为101.4%,100.0%,101.3%。 结论: 该方法操作简便,重复性好,结果准确可靠,适用于该药的质量控制。

Objective: To establish the methods for qualitation and quantitation of Xiongdan pills. Methods: Bear gall,Rhizoma Coptidis,Radix Gentianae and Fructus Gardeniae were identified by TLC;The content of borneol and menthol was determined by GC. The sample was determined with Agilent DB-WAX capillary column(30 m×0.45 mm×0.85 μm) by GC. The detector was FID,N₂ was as carrier gas.The flow rate was 3.0 mL·min^-1;split injection was conducted with split radio of 25∶ 1. Results: Bear gall,Rhizoma Coptidis,Radix Gentianae and Fructus Gardeniae could be identified by TLC;The linear range of borneol was 56.0-672.0 μg·mL^-1and the average recoveries of 3 concentrations(n=3) were 99.4%,101.6%,100.7%,respectively;The linear range of menthol was 56.9-683.3 μg·mL^-1 and the average recoveries of 3 concentrations(n=3) were 101.4%,100.0%,101.3%,respectively. Conclusion: This method is simple,convenient,accurate and repeatable,and can be used for quality control of Xiongdan pills.

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