HPLC法测定胃炎康胶囊中芍药苷、盐酸小檗碱及甘草酸的含量
HPLC analysis of paeoniflorin,berberine hydrochloride and glycyrrhizic acid in Weiyankang capsules
分类号:
出版年·卷·期(页码):2012,32 (1):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------
目的: 以HPLC法同时测定胃炎康胶囊中芍药苷、盐酸小檗碱及甘草酸的含量。 方法: 采用Kromasil C18(250 mm×4.6 mm,5 μm)色谱柱,以乙腈-0.5%三乙胺水溶液(磷酸调pH 3.0)为流动相,梯度洗脱,流速1.0 mL·min-1,检测波长238 nm,柱温30 ℃。 结果: 芍药苷、盐酸小檗碱及甘草酸线性范围分别为21.28~170.2,30.18~241.2,20.86~166.9 mg·L-1(r≥0.9993);平均加样回收率(n=5)分别为97.1%(RSD=1.2%),98.5%(RSD=2.0%),99.7%(RSD=0.87%)。 结论: 该方法操作简便,快速易行,结果准确可靠,可以作为胃炎康胶囊中芍药苷、盐酸小檗碱及甘草酸的含量测定方法。
-----英文摘要:---------------------------------------------------------------------------------------
Objective: To establish an HPLC method for determination of paeoniflorin,berberine hydrochloride and glycyrrhizic acid in Weiyankang capsules. Methods: The separation was performed on a Kromasil C18(250 mm×4.6 mm,5 μm) column;The mobile phase was acetonitrile-0.5% triethylamine aqueous solution(adjusted to pH 3.0 with phosphoric acid) by gradient elution,at the flow rate of 1.0 mL·min-1;The detection wavelength was 238 nm. Results: The linear ranges of paeoniflorin,berberine hydrochloride and glycyrrhizic acid were 21.28-170.2,30.18-241.2,20.86-166.9 mg·L-1(r≥0.9993). The average recoveries(n=5) were 97.1%(RSD=1.2%),98.5%(RSD=2.0%),99.7%(RSD=0.87%),respectively. Conclusion: This method is simple,accurate and reproducible,and can be used for determination of paeoniflorin,berberine hydrochloride and glycyrrhizic acid in Weiyankang capsules
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