反相高效液相色谱法拆分西酞普兰对映异构体
RP-HPLC chiral separation of citalopram
分类号：
出版年·卷·期（页码）：2012,32 (1):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的: 建立西酞普兰对映异构体的HPLC手性分析方法,用于草酸艾司西酞普兰光学纯度的分析测定。 方法: 采用不易变性的卵类粘蛋白键合硅胶(ES-OVM)为手性固定相,以乙腈-0.05 mol·L-1磷酸盐缓冲液(pH 7.0)(15:85)为流动相,流速为1.0 mL·min-1,柱温30 ℃,检测波长240 nm,对西酞普兰对映异构体进行分离测定。 结果: 西酞普兰对映异构体在卵粘蛋白柱上实现了良好的分离,分离度为1.90。将该方法应用于手性拆分得到的草酸艾司西酞普兰的光学纯度测定,结果光学纯度大于97.9%。 结论: 该方法分离度与重现性好,可用于西酞普兰的光学纯度的分析测定及对拆分过程进行监测,结果可靠。
-----英文摘要：---------------------------------------------------------------------------------------
Objective: To establish an HPLC method to separate citalopram enantiomers on ovomucoid chiral column for optical purity detection of escitalopram oxalate. Methods: The mobile phase was recommended as acetonitrile-0.05 mol·L-1 phosphate buffer(pH 7.0)(15:85)at a flow rate of 1.0 mL·min-1.The column temperature was 30 ℃ and the detection wavelength was 240 nm. Results: Citalopram enantiomers were separated under the above conditions,the resolution between S-and R-citalopram was 1.90.The optical purity of escitalopram oxalate was more than 97.9%. Conclusion: With good resolution and repeatability,the method can be reliably used for chiral separation of citalopram enantiomers,and determination of the optical purity of S-and R-citalopram sample.
-----参考文献：---------------------------------------------------------------------------------------
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