HPLC-DAD法测定商品苦参根茎和根中5个生物碱的含量

目的: 测定商品苦参根茎和根中苦参碱、氧化苦参碱、槐果碱、氧化槐果碱、槐定碱的含量,主要了解苦参根茎中苦参碱和氧化苦参碱总量是否达到中国药典2010年版规定的不低于1.0%。 方法: 采用Welch Materials Xtimate^TM C₁₈(4.6 mm×150 mm,5 μm)色谱柱,流动相A为10 mmol·L^-1醋酸铵水溶液(0.1%氨水调至pH 9.2),流动相B为乙腈,梯度洗脱,流速1 mL·min^-1,检测波长220 nm。 结果: 苦参碱进样量在0.1575～2.205 μg(r=0.9999),氧化苦参碱在0.8985～12.58 μg(r=0.9999),槐果碱在0.1360～1.904 μg(r=0.9999),氧化槐果碱在0.4398～6.153 μg(r=0.9998),槐定碱在0.2092～2.926 μg(r=0.9998)范围内呈良好的线性关系;加样平均回收率(n=3)分别为101.0%～102.5%(RSD≤2.1%),100.8%～101.6%(RSD≤2.8%),102.3%～103.6%(RSD≤1.2%),102.2%～103.4%(RSD≤1.1%),102.4%～103.3%(RSD≤2.9%)。 结论: 该方法精密度和准确度均好。苦参根茎和根中苦参碱和氧化苦参碱的总量均不低于1.0%,符合中国药典2010年版的规定。

Objective: To determine the respective content of matrine,oxymatrin,sophocarpine,oxysophocarpine and sophoridine in commercial Kushen's rhizomes and roots so as to find out if the total contents of matrine and oxymatrin meet the request of not less than 1% in ChP(Vol.I)2010 Ed. Methods: The procedure was performed on a C₁₈(4.6 mm×150 mm,5 μm) column with a gradient elution of mobile phase A(10 mmol·L^-1 ammonium acetate aqueous,adjusted to pH 9.2 with ammonia water) and mobile phase B(acetonitrile).The flow rate was 1 mL·min^-1 and the detective wavelength of DAD was set at 220 nm. Results: The linear ranges of matrine,oxymatrin,sophocarpine,oxysophocarpine and sophoridine were 0.1575-2.205 μg(r=0.9999),0.8985-12.58 μg(r=0.9999),0.1360-1.904 μg(r=0.9999),0.4398-6.153 μg(r=0.9998),and 0.2092-2.926 μg(r=0.9998),respectively;The recoveries(n=3) were 101.0%-102.5%(RSD≤2.1%),100.8%-101.6%(RSD≤2.8%),102.3%-103.6%(RSD≤1.2%),102.2%-103.4%(RSD≤1.1%),and 102.4%-103.3%(RSD≤2.9%),respectively. Conclusion: The HPLC method shows a good precision and accuracy.The total contents of matrine and oxymatrine in both Kushen's rhizomes and roots are not less than 1.0%,which meet thestandardsin ChP(Vol.I)2010 Ed.

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