头风痛丸定性定量方法的研究

目的: 建立头风痛丸的定性定量方法,更好地控制产品质量。 方法: 采用薄层色谱法对处方中的3味药材(白芷,川芎,绿茶)进行鉴别,采用高效液相色谱法测定君药白芷中欧前胡素的含量,以甲醇-水(60:40)为流动相,在248 nm波长处检测。 结果: 薄层色谱鉴别方法专属性好,斑点清晰,分离度好,阴性样品无干扰;高效液相色谱法测定,欧前胡素在0.023～0.23 μg范围内线性关系良好(r=0.9999);平均回收率(n=6)为97.7%,RSD=1.9%。 结论: 本方法能准确地进行定性、定量,且专属性强,灵敏度高,重复性好,可用于头风痛丸的质量控制。

Objective: To establish a qualitative and quantitative method of Toufengtong pills for the best quality control. Methods: TLC method was used to identify three herbal medicines(Radix Angelicae Dahuricae;Rhizoma Chuanxiong;Folium Camelliae) in prescription.HPLC method was used to determine the content of imperatorin in Radix Angelicae Dahuricae.The mobile phase was methyl alcohol-water(60:40) and determined at 248 nm. Results: Three herbal medicines in prescription were distinguished exclusively with clear spots and good separation by TLC.For HPLC determination,the imperatorin showed good linear relationship(r=0.9999) in the range of 0.023-0.23 μg,the average recovery(n=6) was 97.7% with RSD of 1.9%. Conclusion: This method has a strong specificity,high sensitivity and fine conclusion and can be used in qualitative and quantitative analysis accurately for the quality control of Toufengtong pills.