HPLC法测定山茱萸中科罗索酸的含量
HPLC determination of corosolic acid in Corni Fructus
分类号：
出版年·卷·期（页码）：2011,31 (12):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的: 建立高效液相色谱法测定山茱萸中科罗索酸的含量。 方法: 采用Inertsil ODS-3(150 mm×4.6 mm,5 μm)色谱柱,流动相为甲醇-水-磷酸(82:18:0.2),流速1.0 mL·min-1,检测波长205 nm,柱温30 ℃。 结果: 科罗索酸进样浓度在59.86～598.6 μg·mL-1范围内与峰面积呈良好的线性关系,平均回收率(n=6)为98.7%(RSD=1.0%)。 结论: 该方法分离度好,专属性强,重复性好,简便易行,可用于山茱萸中科罗索酸的含量测定。
-----英文摘要：---------------------------------------------------------------------------------------
Objective: To establish an HPLC method for determination of corosolic acid in Corni Fructus. Methods: A Inertsil ODS-3(150 mm×4.6 mm,5 μm) column was adopted;The mobile phase consisted of methanol-distilled water-phosphoric acid(82:18:0.2) at a flow rate of 1.0 mL·min-1;The detection wavelenth was 205 nm,and the column temperature was 30 ℃. Results: The standard curves of corosolic acid shows a good linearity in the concentration range of 59.86-598.6 μg·mL-1,and the average recovery(n=6) was 98.7% with RSD of 1.0%. Conclusion: This method is simple,repeatable,and has good resolution and high selectivity.It can be used for the determination of corosolic acid in Corni Fructus.
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