HPLC波长切换法同时测定白芍饮片中9个成分的含量

目的: 建立高效液相色谱波长切换法对白芍中9个成分(没食子酸、氧化芍药苷、儿茶素、芍药内酯苷、芍药苷、苯甲酸、1,2,3,4,6-五没食子酰葡萄糖、苯甲酰芍药苷、丹皮酚)进行分析。 方法: 采用Phenomsil ODS(250 mm×4.6 mm,5 μm)色谱柱,以乙腈(A)-0.1%磷酸水(B)为流动相,梯度洗脱,流速1.0 mL·min^-1,检测波长为267 nm(0～12 min,测定没食子酸)、258 nm(12～30 min,测定氧化芍药苷、儿茶素)、230 nm(30～38 min,测定芍药内酯苷、芍药苷)、223 nm(38～42 min,测定苯甲酸)、275 nm(42～56 min,测定1,2,3,4,6-五没食子酰葡萄糖)、230 nm(56～70 min,测定苯甲酰芍药苷、丹皮酚)。 结果: 白芍中9个成分没食子酸、氧化芍药苷、儿茶素、芍药内酯苷、芍药苷、苯甲酸、1,2,3,4,6-五没食子酰葡萄糖、苯甲酰芍药苷、丹皮酚进样量分别在0.13～0.87 μg(r=0.9991),0.13～0.85 μg(r=0.9991),2.50×10^-3～17.50×10^-3 μg(r=0.9993),0.26～1.78 μg(r=0.9995),0.46～3.20 μg(r=0.9991),0.83×10^-3～5.77×10^-3 μg(r=0.9997),0.28～1.92 μg(r=0.9994),11.00×10^-3～77.00×10^-3 μg(r=0.9994),5.88×10^-3～41.12×10^-3(r=0.9994) μg范围内呈良好线性关系;平均回收率(n=5)分别为98.7%,96.7%,98.0%,97.8%,98.9%,98.3%,97.6%,96.7%,96.7%。 结论: 该方法准确可靠、重复性好,可用于白芍的质量控制。

Objective: To develop an HPLC method for determination of 9 indicative components(gallic acid,hydroxyl-paeoniflorin,catechin,albiflorin,paeoniflorin,1,2,3,4,6-pentagalloylglucose,benzoic acid,benzoyl-paeoniflorin,paeonol) in white peony root. Methods: Phenomsil(250 mm×4.6 mm,5 μm) was adopted;the mobile phase was acetonitrile(A)-0.1% phosphoric acid solution(B) with gradient elution at a flow rate of 1.0 mL·min^-1,and the detection wavelength was 267 nm(0-12 min) for gallic acid,258 nm(12-30 min) for hydroxyl-paeoniflorin and catechin,230 nm(30-38 min) for albiflorin and paeoniflorin,223 nm(38-42 min) for benzoic acid,275 nm(42-56 min) for 1,2,3,4,6-pentagalloylglucose,230 nm(56-70 min) for benzoyl-paeoniflorin and paeonol. Results: The content of 9 indicative components in white peony root was stable.The method had a good linearity in the ranges of 0.13-0.87 μg(r=0.9991) for gallic acid,0.13-0.85 μg(r=0.9991) for hydroxyl-paeoniflorin,,2.50×10^-3-17.50×10^-3 μg(r=0.9993) for catechin,0.26-1.78 μg(r=0.9995) for albiflorin,0.46-3.20 μg(r=0.9991) for paeoniflorin,0.83×10^-3-5.77×10^-3 μg(r=0.9997) for benzoic acid,0.28-1.92 μg(r=0.9994) for 1,2,3,4,6-pentagalloylglucose,11.00×10^-3-77.00×10^-3 μg(r=0.9994) for benzoyl-paeoniflorin,5.88×10^-3-41.12×10^-3 μg(r=0.9994) for paeonol.The average recoveris(n=5) were 98.7%,96.7%,98.0%,97.8%,98.9%,98.3%,97.6%,96.7%,96.7%,respectively. Conclusion: This method is dependable,simple and practical,and may be use to control quality of white peony root.