GC法测定蛇脂冰肤软膏中薄荷脑和冰片的含量

目的: 采用气相色谱法测定蛇脂冰肤软膏中薄荷脑、冰片的含量。 方法: 样品经正己烷超声处理后,采用 Agilent INNOWAX毛细管色谱柱(30 m×0.25 mm×0.25 μm);FID检测器;色谱条件:柱温:110℃,载气流速1.5 mL·min^-1,进样口温度:250 ℃,检测器温度:300 ℃。进样量:1 μL。 结果: 薄荷脑在0.0762~6.0995 mg·mL^-1范围内线性关系良好(r=0.9999,n=6),龙脑在0.0460375~3.683 mg·mL^-1范围内线性关系良好(r=0.9999,n=6)。薄荷脑的平均加样回收率(n＝9)为101.1%,龙脑的平均加样回收率(n＝9)为101.7%。 结论: 该方法灵敏度高、专属性好、操作简便、重现性好。

Objective: To develop an GC method for the determination of menthol and Borneol in Shezhi Bingfu Ointment. Methods: The column was Agilent INNOWAX(30m×0.25mm×0.25μm),and FID detector under following chromatography condition:column temperature was 110 ℃.The gas flow rate was 1.5 mL·min^-1.Injector temperature was set at 250 ℃ and detector at 300 ℃ respectively. Results: Linear ranges was 0.0762–6.0995mg·mL^-1 for menthol and 0.0460375–3.683mg·mL^-1 for Borneol.The average recovery of menthol was 101.1%(n＝9),and average recovery of Borneol was 101.7%(n＝9). Conclusions: The method is simple,sensitive,accurate,specific and reproducible in the quality control of Shezhi Bingfu Ointment.