毛细管区带电泳-间接紫外检测氨基糖苷类抗生素中硫酸根

目的: 用毛细管区带电泳-间接紫外检测技术检测氨基糖苷类抗生素中硫酸根。 方法: 采用非涂层弹性石英毛细管;背景电解质为含0.2 mmol·L^-1十六烷基三甲基溴化铵的15 mmol·L^-1铬酸水溶液(用三羟甲基氨基甲烷调节pH至8.1);操作电压:-20 kV;检测波长:276 nm(间接检测);电泳过程中在进样端始终外加适当的压力使基线稳定。 结果: 本文方法的线性范围为45~150 μg·mL^-1;定量限约为6 μg·mL^-1;硫酸根与内标峰面积比的RSD为0.6%(n=10);测定结果与采用英国药典方法的测定结果一致。 结论: 本方法适用于对氨基糖苷类抗生素中的硫酸根的测定。

Objective: To establish a capillary zone electrophoresis with indirect UV detection method for determination sulfate in aminoglycoside antibiotics. Method: Best results were achieved with the background electrolyte prepared by titrating of 15 mmol·L^-1 chromic acid with tris(hydroxymethyl)methylamine to pH 8.1 and 0.2 mmol·L^-1 cetyltrimethylammonium bromide was added finally and an applied voltage of-20 kV in a bare fused-silica capillary.Indirect UV detection was performed at a wavelength of 276 nm.The application of a small amount of inlet pressure during the separation assisted the attainment of a stable baseline. Resuls: The linear range for sulfate was 45-150 μg·mL^-1.The limit of quantitation was around 6 μg·mL^-1.The RSD for peak area ratio (the area of sulfate peak,divided by the area of the internal standard peak) was 0.6%(n=10).The results were agreed with those obtained with the method of British Pharmacopoeia. Conclusion: The proposed method is suitable for determination of sulfate in aminoglycoside;antibiotics.