角度激光光散射仪和体积排阻色谱法联用检测双黄连注射液中的高分子物质

目的: 多角度激光光散射仪与体积排阻色谱法联用(MALLS-SEC)测定双黄连注射液中的高分子物质。 方法: 以0.7% Na₂SO₄缓冲液(加0.05%NaN₃)为流动相,流速0.5 mL·min^-1,TSK-GEL G3000SWxl凝胶排阻色谱柱与多角度激光光散射仪和示差折光检测器联用。 结果: 该方法对相对分子质量4600~133800 g·mol^-1范围内物质线性关系良好(r=0.9922)。 结论: 本方法简便、快速、准确。

Objective: To determine the macromolecules in Shuanghuanglian injection by the combination of multiangle laser light scattering instrument and size exclusion chromatography (MALLS-SEC). Method: A gel size-exelusion chromatography in conjunction with refractive index detector and multi-angle laser lights scattering instrument,was performed on TSK-Gel G3000SWxl colunm,using 0.7% Na₂SO₄ buffer (added 0.05% NaN₃) as a mobile phase system,and the flow rate was 0.5 mL·min^-1. Results: The calibration curve was linear in the range of 4600-133800 g·mol^-1(r=0.9922). Conclusion: The method is simple,rapid and accurate and can be applied to determine the macromolecules in Shuanghuanglian injection.