在线微生物浊度法测定多维元素片中微量维生素B₁₂的含量

目的: 建立在线微生物浊度法测定多维元素片中微量维生素B₁₂的含量。方法: 以莱士曼氏乳酸杆菌为实验菌,以缺乏维生素B₁₂的培养基为检测培养基,采用浊度法在线检测。结果: 维生素B₁₂浓度在0.02~0.10 ng·mL^-1范围内呈现良好线性关系(r=0.9985),平均回收率为101.8%。结论: 该方法快速、简便、灵敏,在线检测结果准确可靠,适用于多维元素片中微量维生素B₁₂的含量测定。
 

Objective: To establish a method for the determination of trace vitamin B₁₂ in multivitamin formula with minerals tablets. Methods: The test organism was Lactobacillus leichmamii. The medium without vitamin B₁₂ was used as assay medium and measured by on-line microbial turbidimetric method. Results: Vitamin B₁₂ curves were linear in the range of 0.02-0.10 ng·mL^-1(r=0.9985), and the average recovery was 101.8%. Conclusion: This rapid and simple method shows high sensitivity, which is suitable for the assay of trace vitamin B₁₂ in multivitamin formula with minerals tablets. The results are accurate and reliable by on-line method.