HPLC法同时测定藿香正气胶囊中8个主要成分的含量

目的: 建立同时测定藿香正气胶囊中甘草苷、柚皮苷、橙皮苷、麝香草酚、欧前胡素、和厚朴酚、异欧前胡素及厚朴酚共8个主要化学成分的HPLC分析方法。方法: 采用C₁₈(250 mm×4.6 mm,5 μm)色谱柱,流动相为水-甲醇-乙腈,梯度洗脱(0.01~20 min,68∶30∶2→60∶38∶2;20~50 min,60∶38∶2→34∶64∶2;50~65 min,34∶64∶2;65~75 min,34∶64∶2→28∶70∶2;75~85 min,28∶70∶2→68∶30∶2),流速1.0 mL·min^-1,检测波长283 nm。结果: 在各自的线性范围内甘草苷、柚皮苷、橙皮苷、麝香草酚、欧前胡素、和厚朴酚、异欧前胡素、厚朴酚8个化合物的标准曲线呈良好的线性关系;平均加样回收率分别为97.4%,98.5%,97.4%,98.6%,97.8%,99.2%,97.0%,97.5%;所有化合物的精密度和重复性试验的RSD均＜3.0%。结论: 该分析方法简单、灵敏、准确,重复性好,可同时分析藿香正气胶囊中的主要成分。

Objective: To establish an HPLC method for determination of eight main components (liquiritin,naringin,hesperidin,thymol,imperatorin,honokiol,isoimperatorin,and magnolol) in the traditional Chinese medicinal preparation Huoxiang Zhengqi capsules. Methods: The separation was performed on a C₁₈ (250 mm×4.6 mm,5 μm) column by gradient elution with water–methanol–acetonitrile(0.01-20 min,68∶ 30∶ 2→60∶ 38∶ 2;20-50 min,60∶ 38∶ 2→34∶ 64∶ 2;50-65 min,34∶ 64∶ 2;65-75 min,34∶ 64∶ 2→28∶ 70∶ 2;75-85 min,28∶ 70∶ 2→68∶ 30∶ 2) as the mobile phase at a flow rate of 1 mL·min^-1,with UV detection at 283 nm. Results: Eight regression equations showed good linear relationships between the peak area ratio of each marker to internal standard and amounts.The recoveries of the markers listed above were 97.4%,98.5%,97.4%,98.6%,97.8%,99.2%,97.0%,and 97.5%,respectively.The RSDs of precision and repeatability test were less than 3.0%. Conclusion: The method is simple,sensitive and reliable.It can be used for quantitative determination of Huoxiang Zhengqi capsules.