一测多评法测定藏药当佐中没食子酸、羟基红花黄色素-A、桂皮醛及胡椒碱的含量

目的: 建立藏药当佐一测多评法,考察不同类型化合物之间采用相对校正因子进行含量测定的准确性和可行性。方法: 以藏药当佐为研究对象,通过建立胡椒碱与其他3个指标成分间的相对校正因子(RCF),并利用校正因子对没食子酸、羟基红花黄色素-A、桂皮醛的含量进行计算,实现一测多评(计算法);同时采用外标法测定当佐中该4个指标成分含量(实测法),并比较计算值与实测值之间的差异。结果: 在一定的线性范围内,胡椒碱与没食子酸、羟基红花黄色素-A、桂皮醛间的RCF分别为1.3686,0.2620,3.1333;且在不同实验条件下重现性良好(RSD分别为3.7%,2.2%,2.1%);不同来源当佐中4个成分含量的计算值与实测值间无明显差异(RSD<5%)。结论: 本研究建立的只用1个对照品同步测定当佐中4个成分的一测多评法是准确的、可行的,可用于不同类型化合物间的含量测定,为民族药物多指标质量评价提供新的思路。

Objective: To establish a quantitative assay of multi-components by single-marker(QAMS) for determination of four bioactive components in Tibetan medicine Dangzuo,and examine the feasibility of using the relative correction factors(RCFs) between the different types of compound.Methods: Piperine was selected as the internal reference substance,RCFs of gallic acid, hydroxysafflor yellow A and cinnamic aldehyde were calculated. The contents of four components in four samples were determined by both external standard method and QAMS. The validity of the QAMS method was evaluated by comparison of their quantitative results of both methods.Results: RCFs of gallic acid, hydroxysafflor yellow A and cinnamic aldehyde with reference to piperine were 1.3686, 0.2620 and 3.1333 respectively, and repeatability was good in different experimental conditions(RSDs were 3.7%, 2.2% and 2.1%, respectively); the quantitative results of both external standard method and QAMS had no significant difference(RSD<5%). Conclusion: The method established in this research is accurate and feasible that it just need to assay single-marker for determination of four bioactive components in Dangzuo simultaneously. Therefore, this method is suited to determine different types of components, and can provide a new mind for the quality assess of traditional medicine.